In vitro effects of cobalt ions on CNS derived cell lines

Research output: Contribution to conferencePoster

Abstract

Several neurological symptoms associated with the toxic action of cobalt ions have been reported among patients with metal-on-metal (MoM) hip prostheses made of cobalt chromium (CoCr) alloy. The sporadic nature of these manifestations, combined with the fact that the medical evidence is relatively new, have contributed to poor understanding of the impact of cobalt poisoning on the brain. In the present study, we characterise the cytotoxicity of cobalt ions in human U 373 astrocytoma and SH-SY5Y neuroblastoma cell lines. Metal ion uptake with different cobalt chloride concentrations (0 to 500μM) for three time-points (24, 48 and 72h) was measured using inductively coupled plasma mass spectrometry (ICP-MS), while cell viability was tested with MTT and Neutral Red (NR) assays, and by microscopy. The results show that cobalt uptake is dose and time-dependent (up to 415.23, and 69.47μg/L for astrocytes and neurons, respectively), which corresponds with the significant decrease in cell viability (p<.05) at high cobalt concentrations both for the MTT and NR assays. IC50 values were 438.27±37.73 and 267.36±14.57μM at 48h for astrocytes and neurons, respectively (similar values for 72h), with the MTT assay more sensitive at detecting toxicity, suggesting involvement of redox mechanisms. Morphological changes, such as extensive vacuolization of the cytosol, usually associated with autophagy, were observed in the course of cell death. These results indicate that exposure to cobalt at high concentrations could have deleterious effects on brain cells. Future focus will be on the mechanism(s) responsible for cobalt uptake, which may provide a therapeutic intervention for MoM patients.

Conference

ConferenceBritish Toxicology Society Congress 2017
CountryUnited Kingdom
CityLiverpool
Period3/04/175/04/17
Internet address

Fingerprint

Cobalt
Cells
Ions
Assays
Metals
Neurons
Brain
Hip prostheses
Cobalt alloys
Inductively coupled plasma mass spectrometry
Chromium alloys
Cell death
Cytotoxicity
Metal ions
Toxicity
Microscopic examination

Keywords

  • metal on metal
  • cobalt ions
  • hip prostheses

Cite this

Gomez Arnaiz, S., Tate, R., Currie, S., & Grant, M. (2017). In vitro effects of cobalt ions on CNS derived cell lines. Poster session presented at British Toxicology Society Congress 2017, Liverpool, United Kingdom.
Gomez Arnaiz, Sara ; Tate, Rothwelle ; Currie, Susan ; Grant, Mary. / In vitro effects of cobalt ions on CNS derived cell lines. Poster session presented at British Toxicology Society Congress 2017, Liverpool, United Kingdom.1 p.
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abstract = "Several neurological symptoms associated with the toxic action of cobalt ions have been reported among patients with metal-on-metal (MoM) hip prostheses made of cobalt chromium (CoCr) alloy. The sporadic nature of these manifestations, combined with the fact that the medical evidence is relatively new, have contributed to poor understanding of the impact of cobalt poisoning on the brain. In the present study, we characterise the cytotoxicity of cobalt ions in human U 373 astrocytoma and SH-SY5Y neuroblastoma cell lines. Metal ion uptake with different cobalt chloride concentrations (0 to 500μM) for three time-points (24, 48 and 72h) was measured using inductively coupled plasma mass spectrometry (ICP-MS), while cell viability was tested with MTT and Neutral Red (NR) assays, and by microscopy. The results show that cobalt uptake is dose and time-dependent (up to 415.23, and 69.47μg/L for astrocytes and neurons, respectively), which corresponds with the significant decrease in cell viability (p<.05) at high cobalt concentrations both for the MTT and NR assays. IC50 values were 438.27±37.73 and 267.36±14.57μM at 48h for astrocytes and neurons, respectively (similar values for 72h), with the MTT assay more sensitive at detecting toxicity, suggesting involvement of redox mechanisms. Morphological changes, such as extensive vacuolization of the cytosol, usually associated with autophagy, were observed in the course of cell death. These results indicate that exposure to cobalt at high concentrations could have deleterious effects on brain cells. Future focus will be on the mechanism(s) responsible for cobalt uptake, which may provide a therapeutic intervention for MoM patients.",
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note = "British Toxicology Society Congress 2017 ; Conference date: 03-04-2017 Through 05-04-2017",
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Gomez Arnaiz, S, Tate, R, Currie, S & Grant, M 2017, 'In vitro effects of cobalt ions on CNS derived cell lines' British Toxicology Society Congress 2017, Liverpool, United Kingdom, 3/04/17 - 5/04/17, .

In vitro effects of cobalt ions on CNS derived cell lines. / Gomez Arnaiz, Sara; Tate, Rothwelle; Currie, Susan; Grant, Mary.

2017. Poster session presented at British Toxicology Society Congress 2017, Liverpool, United Kingdom.

Research output: Contribution to conferencePoster

TY - CONF

T1 - In vitro effects of cobalt ions on CNS derived cell lines

AU - Gomez Arnaiz, Sara

AU - Tate, Rothwelle

AU - Currie, Susan

AU - Grant, Mary

PY - 2017/4/3

Y1 - 2017/4/3

N2 - Several neurological symptoms associated with the toxic action of cobalt ions have been reported among patients with metal-on-metal (MoM) hip prostheses made of cobalt chromium (CoCr) alloy. The sporadic nature of these manifestations, combined with the fact that the medical evidence is relatively new, have contributed to poor understanding of the impact of cobalt poisoning on the brain. In the present study, we characterise the cytotoxicity of cobalt ions in human U 373 astrocytoma and SH-SY5Y neuroblastoma cell lines. Metal ion uptake with different cobalt chloride concentrations (0 to 500μM) for three time-points (24, 48 and 72h) was measured using inductively coupled plasma mass spectrometry (ICP-MS), while cell viability was tested with MTT and Neutral Red (NR) assays, and by microscopy. The results show that cobalt uptake is dose and time-dependent (up to 415.23, and 69.47μg/L for astrocytes and neurons, respectively), which corresponds with the significant decrease in cell viability (p<.05) at high cobalt concentrations both for the MTT and NR assays. IC50 values were 438.27±37.73 and 267.36±14.57μM at 48h for astrocytes and neurons, respectively (similar values for 72h), with the MTT assay more sensitive at detecting toxicity, suggesting involvement of redox mechanisms. Morphological changes, such as extensive vacuolization of the cytosol, usually associated with autophagy, were observed in the course of cell death. These results indicate that exposure to cobalt at high concentrations could have deleterious effects on brain cells. Future focus will be on the mechanism(s) responsible for cobalt uptake, which may provide a therapeutic intervention for MoM patients.

AB - Several neurological symptoms associated with the toxic action of cobalt ions have been reported among patients with metal-on-metal (MoM) hip prostheses made of cobalt chromium (CoCr) alloy. The sporadic nature of these manifestations, combined with the fact that the medical evidence is relatively new, have contributed to poor understanding of the impact of cobalt poisoning on the brain. In the present study, we characterise the cytotoxicity of cobalt ions in human U 373 astrocytoma and SH-SY5Y neuroblastoma cell lines. Metal ion uptake with different cobalt chloride concentrations (0 to 500μM) for three time-points (24, 48 and 72h) was measured using inductively coupled plasma mass spectrometry (ICP-MS), while cell viability was tested with MTT and Neutral Red (NR) assays, and by microscopy. The results show that cobalt uptake is dose and time-dependent (up to 415.23, and 69.47μg/L for astrocytes and neurons, respectively), which corresponds with the significant decrease in cell viability (p<.05) at high cobalt concentrations both for the MTT and NR assays. IC50 values were 438.27±37.73 and 267.36±14.57μM at 48h for astrocytes and neurons, respectively (similar values for 72h), with the MTT assay more sensitive at detecting toxicity, suggesting involvement of redox mechanisms. Morphological changes, such as extensive vacuolization of the cytosol, usually associated with autophagy, were observed in the course of cell death. These results indicate that exposure to cobalt at high concentrations could have deleterious effects on brain cells. Future focus will be on the mechanism(s) responsible for cobalt uptake, which may provide a therapeutic intervention for MoM patients.

KW - metal on metal

KW - cobalt ions

KW - hip prostheses

UR - http://www.thebts.org/bts-annual-congress-2017/

UR - http://www.thebts.org/

M3 - Poster

ER -

Gomez Arnaiz S, Tate R, Currie S, Grant M. In vitro effects of cobalt ions on CNS derived cell lines. 2017. Poster session presented at British Toxicology Society Congress 2017, Liverpool, United Kingdom.