In-situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation

S. Alison Arnold, J. Crowley, N. Woods, L.M. Harvey, B. McNeil

Research output: Contribution to journalArticle

93 Citations (Scopus)

Abstract

The use of in-situ near infrared spectroscopy (NIRS) as a tool for monitoring four key analytes in a CHO-K1 animal cell culture was investigated. Previous work using on-line NIRS to monitor bioprocesses has involved its application ex-situ where the analyzer is physically outside the fermentor, or to microbial bioprocesses. This novel application of NIRS to monitor analytes within an animal cell culture using a steam sterilizable in-situ fiber optic probe is very important for furthering the use of NIRS within the bioprocessing industry. The method of calibration used to develop the models involved the use of large data sets so that all likely variation in stoichiometry was incorporated within the models. Successful models for glucose, lactate, glutamine, and ammonia were built with Standard Error of Predictions (SEP's) of 0.072 (g/L), 0.0144 (g/L), 0.308 (mM), and 0.036 (mM), respectively of the total concentration range.
LanguageEnglish
Pages13-19
Number of pages6
JournalBiotechnology and Bioengineering
Volume84
Issue number1
DOIs
Publication statusPublished - 2003

Fingerprint

Near infrared spectroscopy
Near-Infrared Spectroscopy
Animal cell culture
Cells
Cell Culture Techniques
Steam
Bioreactors
Glutamine
Ammonia
Stoichiometry
Fiber optics
Calibration
Glucose
Lactic Acid
Industry
Monitoring

Keywords

  • CHO cells
  • in-situ
  • NIRS
  • infrared spectroscopy
  • cell cultivation
  • cells
  • biosciences

Cite this

Arnold, S. Alison ; Crowley, J. ; Woods, N. ; Harvey, L.M. ; McNeil, B. / In-situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation. In: Biotechnology and Bioengineering. 2003 ; Vol. 84, No. 1. pp. 13-19.
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In-situ near infrared spectroscopy to monitor key analytes in mammalian cell cultivation. / Arnold, S. Alison; Crowley, J.; Woods, N.; Harvey, L.M.; McNeil, B.

In: Biotechnology and Bioengineering, Vol. 84, No. 1, 2003, p. 13-19.

Research output: Contribution to journalArticle

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