In situ monitoring of the seed stage of a fermentation process using non-invasive NIR spectrometry

A. Nordon, D. Littlejohn, A.S. Dann, P.A. Jeffkins, M.D. Richardson, S.L. Stimpson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Non-invasive NIR spectrometry has been used to monitor in situ the seed stage of a streptomyces fermentation process. The main spectral change occurred at 7263 cm-1 in the 1st derivative spectrum, and from comparison with off-line NIR spectra acquired of components present in the fermentation broth, can be attributed to biomass. The biomass signal was constant for the first 20 h of the seed stage, after which it decreased before increasing again. The time at which the minimum occurred in the NIR profile was either the same or slightly earlier than that at which a maximum in the carbon dioxide evolution rate (CER) occurred. The changes observed for the biomass signal in the NIR spectra can be attributed to growth and then fragmentation of mycelia, which indicates a change in metabolic activity. Hence, it may be possible to use NIR spectrometry in situ to determine the optimum transfer time for the seed stage of a fermentation process. Spectra were also acquired of the final stage of the same fermentation process. The variation in the biomass signal in the NIR spectra was more complicated in the final stage owing to changes in stir rate, and biomass concentration and morphology.
Original languageEnglish
Pages (from-to)660-666
Number of pages6
JournalAnalyst
Volume133
Issue number5
DOIs
Publication statusPublished - 2008

Keywords

  • streptomyces fermentation
  • NIR spectrometry
  • in situ monitoring
  • biomass

Fingerprint Dive into the research topics of 'In situ monitoring of the seed stage of a fermentation process using non-invasive NIR spectrometry'. Together they form a unique fingerprint.

  • Cite this