In situ detection of pterins by SERS

Ross Stevenson, Robert J. Stokes, Donna MacMillan, David Armstrong, Karen Faulds, Roger Wadsworth, Suma Kununthur, Colin J. Suckling, Duncan Graham

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Surface enhanced Raman scattering (SERS) has been used to detect specific pterin molecules at sub-nanomolar concentrations. SERS is fast becoming a widely used technique for the sensitive and specific detection of multiple analytes. The information-rich and concentration-dependent spectra obtained from SERS make the technique ideally placed for high speed, low cost analysis of almost any analyte. Further, to show the feasibility of SERS in the detection of biologically relevant targets, a synthetic pterin analogue of the naturally occurring pterin cofactor, tetrahydrobiopterin, has been detected at a series of concentrations and the method used for the successful detection of the synthetic pterin in mouse serum. In this analysis, spectroscopic collection was optimized for water-based pteridine derivatives using two visible wavelengths of excitation (514.5 and 632.8 nm) and differing mesoscopic metal nanoparticles allowing the limits of detection to be calculated.
LanguageEnglish
Pages1561-1564
Number of pages3
JournalAnalyst
Volume134
DOIs
Publication statusPublished - Aug 2009

Fingerprint

Pterins
Raman Spectrum Analysis
Raman scattering
scattering
Pteridines
Metal Nanoparticles
Spectroscopic analysis
cost analysis
Metal nanoparticles
Limit of Detection
serum
Derivatives
wavelength
Costs and Cost Analysis
Wavelength
Molecules
in situ
detection
Water
metal

Keywords

  • in situ detection
  • SERS
  • pharmacology
  • biomedical sciences
  • pterins

Cite this

Stevenson, R., Stokes, R. J., MacMillan, D., Armstrong, D., Faulds, K., Wadsworth, R., ... Graham, D. (2009). In situ detection of pterins by SERS. Analyst, 134, 1561-1564. https://doi.org/10.1039/b905562b
Stevenson, Ross ; Stokes, Robert J. ; MacMillan, Donna ; Armstrong, David ; Faulds, Karen ; Wadsworth, Roger ; Kununthur, Suma ; Suckling, Colin J. ; Graham, Duncan. / In situ detection of pterins by SERS. In: Analyst. 2009 ; Vol. 134. pp. 1561-1564.
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Stevenson, R, Stokes, RJ, MacMillan, D, Armstrong, D, Faulds, K, Wadsworth, R, Kununthur, S, Suckling, CJ & Graham, D 2009, 'In situ detection of pterins by SERS' Analyst, vol. 134, pp. 1561-1564. https://doi.org/10.1039/b905562b

In situ detection of pterins by SERS. / Stevenson, Ross; Stokes, Robert J.; MacMillan, Donna; Armstrong, David; Faulds, Karen; Wadsworth, Roger; Kununthur, Suma; Suckling, Colin J.; Graham, Duncan.

In: Analyst, Vol. 134, 08.2009, p. 1561-1564.

Research output: Contribution to journalArticle

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Stevenson R, Stokes RJ, MacMillan D, Armstrong D, Faulds K, Wadsworth R et al. In situ detection of pterins by SERS. Analyst. 2009 Aug;134:1561-1564. https://doi.org/10.1039/b905562b