In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives

Hikmate Abriouel, Beatriz Pérez Montoro, María del Carmen Casado Muñoz, Charles W. Knapp, Antonio Gálvez, Nabil Benomar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lactobacillus pentosus MP-10, isolated from brines of naturally fermented Aloreña green table olives, exhibited high probiotic potential. The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism’s ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4-12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. Bioinformatic analyses revealed L. pentosus MP-10 to be absent of acquired antibiotic resistance genes, and most resistance genes were related to efflux mechanisms; no virulence determinants were found in the genome. This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations.
LanguageEnglish
Article numbere0176801
Number of pages26
JournalPLoS One
Volume12
Issue number6
DOIs
Publication statusPublished - 26 Jun 2017

Fingerprint

food defense
Lactobacillus pentosus
Food safety
Brines
Food Safety
Probiotics
Olea
defense mechanisms
Computer Simulation
probiotics
food safety
Genes
Genome
genomics
genome
Clustered Regularly Interspaced Short Palindromic Repeats
Microbial Drug Resistance
genes
antibiotic resistance
Virulence

Keywords

  • Aloreña table olives
  • lactobacillus pentosus
  • probiotics
  • in silico analysis
  • mobile genetic element
  • antibiotic resistance
  • virulence

Cite this

@article{9272fa2519694b7b9db8623c87a37d74,
title = "In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Alore{\~n}a green table olives",
abstract = "Lactobacillus pentosus MP-10, isolated from brines of naturally fermented Alore{\~n}a green table olives, exhibited high probiotic potential. The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism’s ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4-12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. Bioinformatic analyses revealed L. pentosus MP-10 to be absent of acquired antibiotic resistance genes, and most resistance genes were related to efflux mechanisms; no virulence determinants were found in the genome. This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations.",
keywords = "Alore{\~n}a table olives, lactobacillus pentosus, probiotics, in silico analysis, mobile genetic element, antibiotic resistance, virulence",
author = "Hikmate Abriouel and {P{\'e}rez Montoro}, Beatriz and Mu{\~n}oz, {Mar{\'i}a del Carmen Casado} and Knapp, {Charles W.} and Antonio G{\'a}lvez and Nabil Benomar",
year = "2017",
month = "6",
day = "26",
doi = "10.1371/journal.pone.0176801",
language = "English",
volume = "12",
journal = "PLOS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives. / Abriouel, Hikmate; Pérez Montoro, Beatriz; Muñoz, María del Carmen Casado; Knapp, Charles W.; Gálvez, Antonio; Benomar, Nabil.

In: PLoS One, Vol. 12, No. 6, e0176801, 26.06.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - In silico genomic insights into aspects of food safety and defense mechanisms of a potentially probiotic Lactobacillus pentosus MP-10 isolated from brines of naturally fermented Aloreña green table olives

AU - Abriouel, Hikmate

AU - Pérez Montoro, Beatriz

AU - Muñoz, María del Carmen Casado

AU - Knapp, Charles W.

AU - Gálvez, Antonio

AU - Benomar, Nabil

PY - 2017/6/26

Y1 - 2017/6/26

N2 - Lactobacillus pentosus MP-10, isolated from brines of naturally fermented Aloreña green table olives, exhibited high probiotic potential. The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism’s ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4-12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. Bioinformatic analyses revealed L. pentosus MP-10 to be absent of acquired antibiotic resistance genes, and most resistance genes were related to efflux mechanisms; no virulence determinants were found in the genome. This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations.

AB - Lactobacillus pentosus MP-10, isolated from brines of naturally fermented Aloreña green table olives, exhibited high probiotic potential. The genome sequence of L. pentosus MP-10 is currently considered the largest genome among lactobacilli, highlighting the microorganism’s ecological flexibility and adaptability. Here, we analyzed the complete genome sequence for the presence of acquired antibiotic resistance and virulence determinants to understand their defense mechanisms and explore its safety in food. The annotated genome sequence revealed evidence of diverse mobile genetic elements, such as prophages, transposases and transposons involved in their adaptation to brine-associated niches. In-silico analysis of L. pentosus MP-10 genome sequence identified a CRISPR (clustered regularly interspaced short palindromic repeats)/cas (CRISPR-associated protein genes) as an immune system against foreign genetic elements, which consisted of six arrays (4-12 repeats) and eleven predicted cas genes [CRISPR1 and CRISPR2 consisted of 3 (Type II-C) and 8 (Type I) genes] with high similarity to L. pentosus KCA1. Bioinformatic analyses revealed L. pentosus MP-10 to be absent of acquired antibiotic resistance genes, and most resistance genes were related to efflux mechanisms; no virulence determinants were found in the genome. This suggests that L. pentosus MP-10 could be considered safe and with high-adaptation potential, which could facilitate its application as a starter culture and probiotic in food preparations.

KW - Aloreña table olives

KW - lactobacillus pentosus

KW - probiotics

KW - in silico analysis

KW - mobile genetic element

KW - antibiotic resistance

KW - virulence

UR - http://journals.plos.org/plosone/

U2 - 10.1371/journal.pone.0176801

DO - 10.1371/journal.pone.0176801

M3 - Article

VL - 12

JO - PLOS One

T2 - PLOS One

JF - PLOS One

SN - 1932-6203

IS - 6

M1 - e0176801

ER -