Improving the understanding of oligonucleotide–nanoparticle conjugates using DNA-binding fluorophores

Luca Guerrini, Lee Barrett, Jennifer A. Dougan, Karen Faulds, Duncan Graham

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A novel fluorescent-based method for characterisation of oligonucleotide-nanoparticle conjugates (ONPCs) is reported. We exploit the ability of the double-stranded DNA-binding dye, SYBR Green I, to develop a powerful analytical tool to investigate the melting properties and hybridisation behavior of ONPCs as well as their corresponding DNA-mediated assemblies.

LanguageEnglish
Pages4166-4170
Number of pages5
JournalNanoscale
Volume5
Issue number10
Early online date10 Apr 2013
DOIs
Publication statusPublished - 21 May 2013

Fingerprint

Fluorophores
Oligonucleotides
DNA
Nanoparticles
Melting
Coloring Agents
Dyes
SYBR Green I

Keywords

  • oligonucleotide–nanoparticle conjugates
  • double-stranded DNA
  • binding dye

Cite this

Guerrini, Luca ; Barrett, Lee ; Dougan, Jennifer A. ; Faulds, Karen ; Graham, Duncan. / Improving the understanding of oligonucleotide–nanoparticle conjugates using DNA-binding fluorophores. In: Nanoscale. 2013 ; Vol. 5, No. 10. pp. 4166-4170.
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Improving the understanding of oligonucleotide–nanoparticle conjugates using DNA-binding fluorophores. / Guerrini, Luca; Barrett, Lee; Dougan, Jennifer A.; Faulds, Karen; Graham, Duncan.

In: Nanoscale, Vol. 5, No. 10, 21.05.2013, p. 4166-4170.

Research output: Contribution to journalArticle

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