Improving TCSPC data acquisition from CMOS SPAD arrays

Nikola Krstajić, Simon Poland, David Tyndall, Richard Walker, Simao Coelho, David D U Li, Justin Richardson, Simon Ameer-Beg, Robert Henderson

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

5 Citations (Scopus)

Abstract

We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second). 

LanguageEnglish
Title of host publicationProceedings of SPIE 8797
Subtitle of host publicationAdvanced Microscopy Techniques III
Volume8797
DOIs
Publication statusPublished - 19 Sep 2013
EventAdvanced Microscopy Techniques III - Munich, United Kingdom
Duration: 15 May 201316 May 2013

Conference

ConferenceAdvanced Microscopy Techniques III
CountryUnited Kingdom
CityMunich
Period15/05/1316/05/13

Fingerprint

Photons
data acquisition
Data acquisition
CMOS
counting
photons
Cameras
cameras
acquisition
Microscopes
Fluorescence
microscopes
Convallaria
firmware
life (durability)
fluorescence
Firmware
time functions
Optical Imaging
Pixels

Keywords

  • fluorescence lifetime
  • single photon avalanche photodetector
  • time correlated single photon counting
  • fluorescence lifetime imaging microscopy
  • luminescence
  • microscopes

Cite this

Krstajić, N., Poland, S., Tyndall, D., Walker, R., Coelho, S., Li, D. D. U., ... Henderson, R. (2013). Improving TCSPC data acquisition from CMOS SPAD arrays. In Proceedings of SPIE 8797: Advanced Microscopy Techniques III (Vol. 8797). [879709] https://doi.org/10.1117/12.2032807
Krstajić, Nikola ; Poland, Simon ; Tyndall, David ; Walker, Richard ; Coelho, Simao ; Li, David D U ; Richardson, Justin ; Ameer-Beg, Simon ; Henderson, Robert. / Improving TCSPC data acquisition from CMOS SPAD arrays. Proceedings of SPIE 8797: Advanced Microscopy Techniques III. Vol. 8797 2013.
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abstract = "We present a digital architecture for fast acquisition of time correlated single photon counting (TCSPC) timestamps from 32×32 CMOS SPAD array. Custom firmware was written to select 64 pixels out of 1024 available for fast transfer of TCSPC timestamps. Our 64 channel TCSPC is capable of acquiring up to 10 million TCSPC timestamps per second over a USB2 link. We describe the TCSPC camera (Megaframe), camera interface to the PC and the microscope setup. We characterize the Megaframe camera for fluorescence lifetime imaging (FLIM) including instrument response function, time resolution and variability of both across the array. We show a fluorescence lifetime image of a plant specimen (Convallaria majalis) from a custom-built multifocal multiphoton microscope. The image was acquired in 20 seconds (with average timestamp acquisition rate of 4.7 million counts per second). ",
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Krstajić, N, Poland, S, Tyndall, D, Walker, R, Coelho, S, Li, DDU, Richardson, J, Ameer-Beg, S & Henderson, R 2013, Improving TCSPC data acquisition from CMOS SPAD arrays. in Proceedings of SPIE 8797: Advanced Microscopy Techniques III. vol. 8797, 879709, Advanced Microscopy Techniques III, Munich, United Kingdom, 15/05/13. https://doi.org/10.1117/12.2032807

Improving TCSPC data acquisition from CMOS SPAD arrays. / Krstajić, Nikola; Poland, Simon; Tyndall, David; Walker, Richard; Coelho, Simao; Li, David D U; Richardson, Justin; Ameer-Beg, Simon; Henderson, Robert.

Proceedings of SPIE 8797: Advanced Microscopy Techniques III. Vol. 8797 2013. 879709.

Research output: Chapter in Book/Report/Conference proceedingConference contribution book

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AU - Poland, Simon

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AU - Richardson, Justin

AU - Ameer-Beg, Simon

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Krstajić N, Poland S, Tyndall D, Walker R, Coelho S, Li DDU et al. Improving TCSPC data acquisition from CMOS SPAD arrays. In Proceedings of SPIE 8797: Advanced Microscopy Techniques III. Vol. 8797. 2013. 879709 https://doi.org/10.1117/12.2032807