Improvements in alzheimer's disease diagnosis using principle components analysis (PCA) in combination with raman spectroscopy

J.K.J. Archer; C.D. Sudworth; R.A. Black; Rachel Williams; Thien How; Nicholas Stone; David Mann

doi:10.1117/12.728464

Improvements in alzheimer's disease diagnosis using principle components analysis (PCA) in combination with raman spectroscopy

J.K.J. Archer, C.D. Sudworth, R.A. Black, Rachel Williams, Thien How, Nicholas Stone, David Mann

Research output: Contribution to journal › Article › peer-review

Abstract

The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.

Original language	English
Pages (from-to)	662810
Journal	Proceedings of SPIE the International Society for Optical Engineering
Volume	6628
DOIs	https://doi.org/10.1117/12.728464
Publication status	Published - Jun 2007

Keywords

alzheimer's disease
diagnosis
principle components analysis
raman spectroscopy

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10.1117/12.728464

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title = "Improvements in alzheimer's disease diagnosis using principle components analysis (PCA) in combination with raman spectroscopy",

abstract = "The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.",

keywords = "alzheimer's disease, diagnosis, principle components analysis, raman spectroscopy",

author = "J.K.J. Archer and C.D. Sudworth and R.A. Black and Rachel Williams and Thien How and Nicholas Stone and David Mann",

year = "2007",

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AU - Archer, J.K.J.

AU - Sudworth, C.D.

AU - Black, R.A.

AU - Williams, Rachel

AU - How, Thien

AU - Stone, Nicholas

AU - Mann, David

PY - 2007/6

Y1 - 2007/6

N2 - The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.

AB - The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.

KW - alzheimer's disease

KW - diagnosis

KW - principle components analysis

KW - raman spectroscopy

UR - http://dx.doi.org/10.1117/12.728464

U2 - 10.1117/12.728464

DO - 10.1117/12.728464

M3 - Article

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VL - 6628

SP - 662810

JO - Proceedings of SPIE the International Society for Optical Engineering

JF - Proceedings of SPIE the International Society for Optical Engineering

ER -

Improvements in alzheimer's disease diagnosis using principle components analysis (PCA) in combination with raman spectroscopy

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