The use of optical coherence tomography (OCT) as a monitoring tool in the growth of human fibroblasts cells in collagen-based constructs is investigated. Rat-tail tendon type-1 collagen based gels mixed with human fibroblasts were prepared and incubated. Fixed samples were then imaged using OCT, and subsequently cross-sectioned and analysed microscopically. The concentration of cells in samples under different contraction dynamics was investigated using analysis of the OCT images. Results show clear differences in scattering intensity as a consequence of cell concentration in both OCT images and micrographs.
|Journal||Proceedings of SPIE the International Society for Optical Engineering|
|Publication status||Published - Jun 2007|
- alzheimer's disease
- principle components analysis
- raman spectroscopy
Archer, J. K. J., Sudworth, C. D., Black, R. A., Williams, R., How, T., Stone, N., & Mann, D. (2007). Improvements in alzheimer's disease diagnosis using principle components analysis (PCA) in combination with raman spectroscopy. Proceedings of SPIE the International Society for Optical Engineering, 6628, 662810. https://doi.org/10.1117/12.728464