Improved sectioning in a slit scanning confocal microscope

V. Poher, G.T. Kennedy, H.B. Manning, D.M. Owen, H.X. Zhang, E. Gu, P.M.W. French, M.D. Dawson, M.A.A. Neil

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

We describe a simple implementation of a slit scanning confocal microscope to obtain an axial resolution better than that of a point-scanning confocal microscope. Under slit illumination, images of a fluorescent object are captured using an array detector instead of a line detector so that out-of-focus light is recorded and then subtracted from the adjacent images. Axial resolution after background subtraction is 2.2 times better than the slit confocal resolution, and out-of-focus image suppression is calculated to attenuate with defocus faster by 1 order of magnitude than in the point confocal case.
LanguageEnglish
Pages1813-1815
Number of pages2
JournalOptics Letters
Volume33
Issue number16
DOIs
Publication statusPublished - 6 Aug 2008

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slits
microscopes
scanning
detectors
subtraction
illumination
retarding

Keywords

  • Line scanning confocal microscope
  • axial resolution improvement
  • Physics
  • Photonics
  • Optics.

Cite this

Poher, V., Kennedy, G. T., Manning, H. B., Owen, D. M., Zhang, H. X., Gu, E., ... Neil, M. A. A. (2008). Improved sectioning in a slit scanning confocal microscope. Optics Letters, 33(16), 1813-1815. https://doi.org/10.1364/OL.33.001813
Poher, V. ; Kennedy, G.T. ; Manning, H.B. ; Owen, D.M. ; Zhang, H.X. ; Gu, E. ; French, P.M.W. ; Dawson, M.D. ; Neil, M.A.A. / Improved sectioning in a slit scanning confocal microscope. In: Optics Letters. 2008 ; Vol. 33, No. 16. pp. 1813-1815.
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Poher, V, Kennedy, GT, Manning, HB, Owen, DM, Zhang, HX, Gu, E, French, PMW, Dawson, MD & Neil, MAA 2008, 'Improved sectioning in a slit scanning confocal microscope' Optics Letters, vol. 33, no. 16, pp. 1813-1815. https://doi.org/10.1364/OL.33.001813

Improved sectioning in a slit scanning confocal microscope. / Poher, V.; Kennedy, G.T.; Manning, H.B.; Owen, D.M.; Zhang, H.X.; Gu, E.; French, P.M.W.; Dawson, M.D.; Neil, M.A.A.

In: Optics Letters, Vol. 33, No. 16, 06.08.2008, p. 1813-1815.

Research output: Contribution to journalArticle

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AU - French, P.M.W.

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Poher V, Kennedy GT, Manning HB, Owen DM, Zhang HX, Gu E et al. Improved sectioning in a slit scanning confocal microscope. Optics Letters. 2008 Aug 6;33(16):1813-1815. https://doi.org/10.1364/OL.33.001813