Impaired Gluconeogenesis in a porcine model of paracetamol induced acute liver failure

Konstantinos Dabos, Henry Whalen, Philip Newsome, John Parkinson, Neil Henderson, Ian Sadler, Peter Hayes, John Plevris

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF).

Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using (1)H NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples.

No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05).

Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state.
LanguageEnglish
Pages1457-1461
Number of pages5
JournalWorld Journal of Gastroenterology
Volume17
Issue number11
DOIs
Publication statusPublished - 21 Mar 2011

Fingerprint

Gluconeogenesis
Acute Liver Failure
Acetaminophen
Swine
Pyruvic Acid
Lactic Acid
Anesthesia
Amino Acids
Citric Acid Cycle
Isoleucine
Valine
Threonine
Phenylalanine
Alanine
Glycine
General Anesthesia
Tyrosine
Veins
Homeostasis
Magnetic Resonance Spectroscopy

Keywords

  • lactate
  • pyruvate
  • branch chain amino acids
  • aromatic amino acids

Cite this

Dabos, Konstantinos ; Whalen, Henry ; Newsome, Philip ; Parkinson, John ; Henderson, Neil ; Sadler, Ian ; Hayes, Peter ; Plevris, John. / Impaired Gluconeogenesis in a porcine model of paracetamol induced acute liver failure. In: World Journal of Gastroenterology. 2011 ; Vol. 17, No. 11. pp. 1457-1461.
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abstract = "To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF). Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using (1)H NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples. No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05). Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state.",
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Dabos, K, Whalen, H, Newsome, P, Parkinson, J, Henderson, N, Sadler, I, Hayes, P & Plevris, J 2011, 'Impaired Gluconeogenesis in a porcine model of paracetamol induced acute liver failure' World Journal of Gastroenterology, vol. 17, no. 11, pp. 1457-1461. https://doi.org/10.3748/wjg.v17.i11.1457

Impaired Gluconeogenesis in a porcine model of paracetamol induced acute liver failure. / Dabos, Konstantinos; Whalen, Henry; Newsome, Philip; Parkinson, John; Henderson, Neil; Sadler, Ian; Hayes, Peter ; Plevris, John.

In: World Journal of Gastroenterology, Vol. 17, No. 11, 21.03.2011, p. 1457-1461.

Research output: Contribution to journalArticle

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T1 - Impaired Gluconeogenesis in a porcine model of paracetamol induced acute liver failure

AU - Dabos, Konstantinos

AU - Whalen, Henry

AU - Newsome, Philip

AU - Parkinson, John

AU - Henderson, Neil

AU - Sadler, Ian

AU - Hayes, Peter

AU - Plevris, John

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N2 - To investigate glucose homeostasis and in particular gluconeogenesis in a large animal model of acute liver failure (ALF). Six pigs with paracetamol induced ALF under general anaesthesia were studied over 25 h. Plasma samples were withdrawn every five hours from a central vein. Three animals were used as controls and were maintained under anaesthesia only. Using (1)H NMR spectroscopy we identified most gluconeogenic amino acids along with lactate and pyruvate in the animal plasma samples. No significant changes were observed in the concentrations of the amino acids studied in the animals maintained under anaesthesia only. If we look at the ALF animals, we observed a statistically significant rise of lactate (P < 0.003) and pyruvate (P < 0.018) at the end of the experiments. We also observed statistically significant rises in the concentrations of alanine (P < 0.002), glycine (P < 0.005), threonine (P < 0.048), tyrosine (P < 0.000), phenylalanine (P < 0.000) and isoleucine (P < 0.01). Valine levels decreased significantly (P < 0.05). Our pig model of ALF is characterized by an altered gluconeogenetic capacity, an impaired tricarboxylic acid (TCA) cycle and a glycolytic state.

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KW - pyruvate

KW - branch chain amino acids

KW - aromatic amino acids

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