Immunomodulatory dendritic cells in intestinal lamina propria

F.G. Chirdo, O.R. Millington, H. Beacock-Sharp, A.M. Mowat

Research output: Contribution to journalArticle

180 Citations (Scopus)

Abstract

The lamina propria (LP) of the small intestine contains many dendritic cells (DC), which are likely to be in close contact with luminal antigens, but their role in intestinal immune responses has been overlooked. Here we show that after feeding mice ovalbumin (OVA), the majority of antigen uptake is associated with DC in the small intestinal LP, and we describe the isolation, purification and initial characterization of theses DC. We obtained >90% CD11c+ DC using magnetic cell sorting, of which the majority were CD11b+CD8–, with smaller numbers of CD11b–CD8+ and CD11b–CD8– DC as well as a distinct population of CD11cintclass II MHClo B220+ DC. Freshly isolated LP DC expressed variable but generally low levels of CD40, CD80 and CD86, which were up-regulated by activation with LPS. LP DC were endocytic in vivo and in vitro and could present antigen to OVA-specific CD4+ T cells in vitro. Antigen-loaded LP DC from OVA-fed mice also primed specific CD4+ T cells in vivo and in vitro, but adoptive transfer of these DC into naive recipients induced hyporesponsiveness to subsequent challenge. LP DC also expressed significant levels of mRNA for IL-10 and type I IFN, but not IL-12, suggesting they may play a central and unique role in immune homeostasis in the gut.

LanguageEnglish
Pages1831-1840
Number of pages10
JournalEuropean Journal of Immunology
Volume35
Issue number6
DOIs
Publication statusPublished - 2005

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Dendritic Cells
Mucous Membrane
Ovalbumin
Antigens
T-Lymphocytes
Adoptive Transfer
Interleukin-12
Interleukin-10
Small Intestine
Homeostasis
Messenger RNA

Keywords

  • Dendritic cells
  • oral tolerance
  • Lamina propria
  • Intestinal mucosa

Cite this

Chirdo, F. G., Millington, O. R., Beacock-Sharp, H., & Mowat, A. M. (2005). Immunomodulatory dendritic cells in intestinal lamina propria. European Journal of Immunology, 35(6), 1831-1840. https://doi.org/10.1002/eji.200425882
Chirdo, F.G. ; Millington, O.R. ; Beacock-Sharp, H. ; Mowat, A.M. / Immunomodulatory dendritic cells in intestinal lamina propria. In: European Journal of Immunology. 2005 ; Vol. 35, No. 6. pp. 1831-1840.
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Chirdo, FG, Millington, OR, Beacock-Sharp, H & Mowat, AM 2005, 'Immunomodulatory dendritic cells in intestinal lamina propria' European Journal of Immunology, vol. 35, no. 6, pp. 1831-1840. https://doi.org/10.1002/eji.200425882

Immunomodulatory dendritic cells in intestinal lamina propria. / Chirdo, F.G.; Millington, O.R.; Beacock-Sharp, H.; Mowat, A.M.

In: European Journal of Immunology, Vol. 35, No. 6, 2005, p. 1831-1840.

Research output: Contribution to journalArticle

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T1 - Immunomodulatory dendritic cells in intestinal lamina propria

AU - Chirdo, F.G.

AU - Millington, O.R.

AU - Beacock-Sharp, H.

AU - Mowat, A.M.

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AB - The lamina propria (LP) of the small intestine contains many dendritic cells (DC), which are likely to be in close contact with luminal antigens, but their role in intestinal immune responses has been overlooked. Here we show that after feeding mice ovalbumin (OVA), the majority of antigen uptake is associated with DC in the small intestinal LP, and we describe the isolation, purification and initial characterization of theses DC. We obtained >90% CD11c+ DC using magnetic cell sorting, of which the majority were CD11b+CD8–, with smaller numbers of CD11b–CD8+ and CD11b–CD8– DC as well as a distinct population of CD11cintclass II MHClo B220+ DC. Freshly isolated LP DC expressed variable but generally low levels of CD40, CD80 and CD86, which were up-regulated by activation with LPS. LP DC were endocytic in vivo and in vitro and could present antigen to OVA-specific CD4+ T cells in vitro. Antigen-loaded LP DC from OVA-fed mice also primed specific CD4+ T cells in vivo and in vitro, but adoptive transfer of these DC into naive recipients induced hyporesponsiveness to subsequent challenge. LP DC also expressed significant levels of mRNA for IL-10 and type I IFN, but not IL-12, suggesting they may play a central and unique role in immune homeostasis in the gut.

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KW - oral tolerance

KW - Lamina propria

KW - Intestinal mucosa

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