Immunological castration using a gonadotrophin-releasing hormone analogue conjugated to PPD

V. A. Ferro, J. E. O'Grady, J. Notman, W. H. Stimson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The importance of gonadotrophin-releasing hormone/luteinizing hormone-releasing hormone (GnRH/LHRH) to reproductive function is illustrated when animals immunized with GnRH produce a high humoral immune response which can achieve a humane alternative to surgical castration. In this paper, the male rat was used as a model Rats were immunized with an analogue of GnRH—GnRH-glycys-in both its free form and conjugated to the carrier PPD. Pre-immunization priming with BCG was compared with non-BCG priming. The results showed that BCG priming, in the absence of adjuvant, allowed a sufficient reduction in testosterone levels to occur without severely affecting gonadal changes. Immunization in conjunction with adjuvant resulted in high antibody titres, a decrease in testosterone levels, gonadal atrophy and a reduction in spermatogenesis. Alteration of the dose levels of the analogue and the long-term effects of immunization on gondal function were also investigated.

LanguageEnglish
Pages259-272
Number of pages14
JournalFood and Agricultural Immunology
Volume7
Issue number3
DOIs
Publication statusPublished - 1 Jan 1995

Fingerprint

synthetic hormones
Castration
Tuberculin
gonadotropin-releasing hormone
castration
Gonadotropin-Releasing Hormone
immunization
testosterone
adjuvants
Immunization
Mycobacterium bovis
Testosterone
spermatogenesis
atrophy
humoral immunity
long term effects
animal models
Spermatogenesis
Humoral Immunity
antibodies

Keywords

  • active immunization GnRH analogue
  • gonadal atrophy
  • immunoneutralization
  • PPD conjugate
  • testosterone

Cite this

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Immunological castration using a gonadotrophin-releasing hormone analogue conjugated to PPD. / Ferro, V. A.; O'Grady, J. E.; Notman, J.; Stimson, W. H.

In: Food and Agricultural Immunology, Vol. 7, No. 3, 01.01.1995, p. 259-272.

Research output: Contribution to journalArticle

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