Immunoassay for P38 MAPK using surface enhanced resonance Raman spectroscopy (SERRS)

P. Douglas; R.J. Stokes; D. Graham; W.E. Smith

doi:10.1039/b715824f

Immunoassay for P38 MAPK using surface enhanced resonance Raman spectroscopy (SERRS)

P. Douglas, R.J. Stokes, D. Graham, W.E. Smith

Pure And Applied Chemistry

Research output: Contribution to journal › Article

24 Citations (Scopus)

Abstract

A micro-bead sandwich assay for P38 mitogen-activated protein kinase using surface enhanced resonance Raman spectroscopy (SERRS) detection is reported. Monoclonal capture antibodies were immobilised on a solid phase of magnetic micro-beads with secondary detection using a rhodamine-labelled antibody. Quantitative SERRS detection of the secondary antibody was possible with a limit of detection of 9.5 × 10−12 mol dm−3. The sandwich assay was quantitative and sensitive to 6 ng ml−1. The mechanism of the SERRS detection in the immunoassay was investigated. The addition of SERRS aggregating agents causes the dissociation of the immuno-complex from the magnetic beads. Scanning electron microscopy images indicate that the colloidal suspension rather than adsorbed silver nanoparticles on the beads provide the SERRS signals, that the aggregate size is partially controlled and that there is some inhomogeneity in the distribution of organic matter on the nanoscale. (Abstract from: http://www.swetswise.com/eAccess/viewAbstract.do?articleID=36962529)

Original language	English
Pages (from-to)	791-796
Number of pages	5
Journal	Analyst
Volume	133
Issue number	6
DOIs	https://doi.org/10.1039/b715824f
Publication status	Published - 2008

Keywords

immunoassay
P38 MAPK
surface enhanced
resonance Raman spectroscopy
(SERRS)

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10.1039/b715824f

http://dx.doi.org/10.1039/b715824f

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Projects

1 Finished

NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

Birch, D., Dawson, M., Faulds, K., Girkin, J., Graham, D., Martin, R., O'Donnell, K., Rolinski, O., Smith, W. & Wynne, K.

Scottish Funding Council SFC, EPSRC (Engineering and Physical Sciences Research Council)

1/08/08 → 31/01/12

Project: Research

Cite this

Douglas, P., Stokes, R. J., Graham, D., & Smith, W. E. (2008). Immunoassay for P38 MAPK using surface enhanced resonance Raman spectroscopy (SERRS). Analyst, 133(6), 791-796. https://doi.org/10.1039/b715824f

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