Immunoassay for P38 MAPK using surface enhanced resonance Raman spectroscopy (SERRS)

P. Douglas, R.J. Stokes, D. Graham, W.E. Smith

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

A micro-bead sandwich assay for P38 mitogen-activated protein kinase using surface enhanced resonance Raman spectroscopy (SERRS) detection is reported. Monoclonal capture antibodies were immobilised on a solid phase of magnetic micro-beads with secondary detection using a rhodamine-labelled antibody. Quantitative SERRS detection of the secondary antibody was possible with a limit of detection of 9.5 × 10−12 mol dm−3. The sandwich assay was quantitative and sensitive to 6 ng ml−1. The mechanism of the SERRS detection in the immunoassay was investigated. The addition of SERRS aggregating agents causes the dissociation of the immuno-complex from the magnetic beads. Scanning electron microscopy images indicate that the colloidal suspension rather than adsorbed silver nanoparticles on the beads provide the SERRS signals, that the aggregate size is partially controlled and that there is some inhomogeneity in the distribution of organic matter on the nanoscale. (Abstract from: http://www.swetswise.com/eAccess/viewAbstract.do?articleID=36962529)
Original languageEnglish
Pages (from-to)791-796
Number of pages5
JournalAnalyst
Volume133
Issue number6
DOIs
Publication statusPublished - 2008

Keywords

  • immunoassay
  • P38 MAPK
  • surface enhanced
  • resonance Raman spectroscopy
  • (SERRS)

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