Immunoassay arrays fabricated by dip-pen nanolithography with resonance raman detection

Stacey Laing, Eleanore J. Irvine, Aaron Hernandez-Santana, W. Ewen Smith, Karen Faulds, Duncan Graham

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Here, we report the first use of resonance Raman scattering for the detection of miniaturized microscale arrays fabricated by dip-pen nanolithography. Antibody arrays for prostate-specific antigen (PSA) were printed, and a sandwich immunoassay was carried out. An enzyme-linked detection antibody was used to provide an insoluble and stable colored microdot in the recommended size range for microarray readers, which could be read with resonance Raman scattering. This gives quantitative detection as well as an improved detection limit and a larger dynamic range than that previously achieved by direct fluorescent detection methods. By Raman mapping across the arrayed area, the microdots were easily detected with very little background signal from surrounding areas. Levels of PSA as low as 25 pg/mL were detected using this method, which could be extended to a large number of useful biomarkers.

LanguageEnglish
Pages5617-5621
Number of pages5
JournalAnalytical Chemistry
Volume85
Issue number12
DOIs
Publication statusPublished - 18 Jun 2013

Fingerprint

Nanolithography
Prostate-Specific Antigen
Raman scattering
Antibodies
Biomarkers
Microarrays
Enzymes

Keywords

  • proteomics
  • antibody microarrays
  • cytokines
  • system
  • Elisa
  • performance
  • surfaces
  • protein nanoarrays
  • linked immunosorbent assay

Cite this

Laing, Stacey ; Irvine, Eleanore J. ; Hernandez-Santana, Aaron ; Smith, W. Ewen ; Faulds, Karen ; Graham, Duncan. / Immunoassay arrays fabricated by dip-pen nanolithography with resonance raman detection. In: Analytical Chemistry. 2013 ; Vol. 85, No. 12. pp. 5617-5621.
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Immunoassay arrays fabricated by dip-pen nanolithography with resonance raman detection. / Laing, Stacey; Irvine, Eleanore J.; Hernandez-Santana, Aaron; Smith, W. Ewen; Faulds, Karen; Graham, Duncan.

In: Analytical Chemistry, Vol. 85, No. 12, 18.06.2013, p. 5617-5621.

Research output: Contribution to journalArticle

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