Immunization of Female Mice with a Plasmid DNA Vaccine Coding EightRepeats of Gonadotrophin Releasing Hormone (Gnrh-I) and Eight T-HelperEpitopes Suppress Fertility In Vivo

Umme Rima, Tadashi Kimura, Ayman M Gebril, Mohammad Islam, Abu Bari, Valerie Ferro, Mohammad Khan

Research output: Contribution to journalArticle

Abstract

Induction of an appropriate immune response against gonadotrophin releasing hormone (GnRH-I) disrupt fertility,reduce fecundity and regress tumours of reproductive system.To disrupt fertility a plasmid DNA vaccine wasengineered coding eight repeats of GnRH-I and eight T-helper epitopes. Translation efficiency of the vaccine wasevaluated in undifferentiated COS1 cells and found to release GnRH-I fusion protein in culture supernatant. Swissalbino female mice (N=24) were immunized with 50μg plasmid DNA construct in study weeks 0, 3, 6, 9 and 12.Group 2 mice were primed with the plasmid DNA in hemagglutinating virus of japanese envelope (HVJE) vector andsubsequent boosts were carried out in phosphate buffer saline. Group 3 mice were immunized with the plasmid DNAin non-ionic surfactant vesicles (NISV) and Group 1 was served as untreated control. The effect of immunization wasstudied in terms of anti-GnRH-I antibody response (OD value at A540 ± SD), suppression of ovarian folliculogenesis,altered uterine histoarchitecture and impaired fertility in vivo in mating trials. In study week 24 OD values of anti-GnRH-I antibody response were 0.982 ± 0.231 in Group 3 mice, followed by 0.783 ± 0.191 in Group 2 in comparisonwith no response in Group 1 controls (0.237 ± 0.147). Results of mating trials showed conception failure invaccinated mice; 51, 18 and 05 pups were seen in the uteri of Groups 1, 2 and 3 mice respectively. There wassignificant (p>0.001) reduction in the weight of ovaries in Group 2 (8.50 ± 2.38 mg) and Group 3 (7.25 ± 0.95 mg)mice compared to Group 1 control (15.00 ± 1.41 mg). Significant reduction of ovarian folliculogenesis was seen inGroup 2 (p>0.001) and Group 3 mice (p>0.01). In conclusion, the plasmid DNA vaccine delivered in female micewith HVJE and NISV induced significantly (p>0.001) higher levels of anti-GnRH-I antibody response, suppressedovarian and uterine function and impaired fertility in vivo.
LanguageEnglish
Article number1000282
Number of pages7
JournalJournal of Vaccines and Vaccination
Volume6
Issue number3
DOIs
Publication statusPublished - 26 Mar 2015

Fingerprint

DNA Vaccines
recombinant vaccines
gonadotropin-releasing hormone
Gonadotropin-Releasing Hormone
Fertility
Immunization
plasmids
immunization
Plasmids
mice
Antibody Formation
follicular development
Surface-Active Agents
antibodies
Viruses
viruses
Control Groups
DNA
reproductive system
pups

Keywords

  • mice
  • DNA
  • vaccine
  • GnRH-I
  • in vivo fertility
  • ovarian folliculogenesis

Cite this

@article{6efcd34327664dd8bd43c0e8e6b96e71,
title = "Immunization of Female Mice with a Plasmid DNA Vaccine Coding EightRepeats of Gonadotrophin Releasing Hormone (Gnrh-I) and Eight T-HelperEpitopes Suppress Fertility In Vivo",
abstract = "Induction of an appropriate immune response against gonadotrophin releasing hormone (GnRH-I) disrupt fertility,reduce fecundity and regress tumours of reproductive system.To disrupt fertility a plasmid DNA vaccine wasengineered coding eight repeats of GnRH-I and eight T-helper epitopes. Translation efficiency of the vaccine wasevaluated in undifferentiated COS1 cells and found to release GnRH-I fusion protein in culture supernatant. Swissalbino female mice (N=24) were immunized with 50μg plasmid DNA construct in study weeks 0, 3, 6, 9 and 12.Group 2 mice were primed with the plasmid DNA in hemagglutinating virus of japanese envelope (HVJE) vector andsubsequent boosts were carried out in phosphate buffer saline. Group 3 mice were immunized with the plasmid DNAin non-ionic surfactant vesicles (NISV) and Group 1 was served as untreated control. The effect of immunization wasstudied in terms of anti-GnRH-I antibody response (OD value at A540 ± SD), suppression of ovarian folliculogenesis,altered uterine histoarchitecture and impaired fertility in vivo in mating trials. In study week 24 OD values of anti-GnRH-I antibody response were 0.982 ± 0.231 in Group 3 mice, followed by 0.783 ± 0.191 in Group 2 in comparisonwith no response in Group 1 controls (0.237 ± 0.147). Results of mating trials showed conception failure invaccinated mice; 51, 18 and 05 pups were seen in the uteri of Groups 1, 2 and 3 mice respectively. There wassignificant (p>0.001) reduction in the weight of ovaries in Group 2 (8.50 ± 2.38 mg) and Group 3 (7.25 ± 0.95 mg)mice compared to Group 1 control (15.00 ± 1.41 mg). Significant reduction of ovarian folliculogenesis was seen inGroup 2 (p>0.001) and Group 3 mice (p>0.01). In conclusion, the plasmid DNA vaccine delivered in female micewith HVJE and NISV induced significantly (p>0.001) higher levels of anti-GnRH-I antibody response, suppressedovarian and uterine function and impaired fertility in vivo.",
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Immunization of Female Mice with a Plasmid DNA Vaccine Coding EightRepeats of Gonadotrophin Releasing Hormone (Gnrh-I) and Eight T-HelperEpitopes Suppress Fertility In Vivo. / Rima, Umme; Kimura, Tadashi; Gebril, Ayman M; Islam, Mohammad; Bari, Abu; Ferro, Valerie; Khan, Mohammad.

In: Journal of Vaccines and Vaccination, Vol. 6, No. 3, 1000282, 26.03.2015.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immunization of Female Mice with a Plasmid DNA Vaccine Coding EightRepeats of Gonadotrophin Releasing Hormone (Gnrh-I) and Eight T-HelperEpitopes Suppress Fertility In Vivo

AU - Rima, Umme

AU - Kimura, Tadashi

AU - Gebril, Ayman M

AU - Islam, Mohammad

AU - Bari, Abu

AU - Ferro, Valerie

AU - Khan, Mohammad

PY - 2015/3/26

Y1 - 2015/3/26

N2 - Induction of an appropriate immune response against gonadotrophin releasing hormone (GnRH-I) disrupt fertility,reduce fecundity and regress tumours of reproductive system.To disrupt fertility a plasmid DNA vaccine wasengineered coding eight repeats of GnRH-I and eight T-helper epitopes. Translation efficiency of the vaccine wasevaluated in undifferentiated COS1 cells and found to release GnRH-I fusion protein in culture supernatant. Swissalbino female mice (N=24) were immunized with 50μg plasmid DNA construct in study weeks 0, 3, 6, 9 and 12.Group 2 mice were primed with the plasmid DNA in hemagglutinating virus of japanese envelope (HVJE) vector andsubsequent boosts were carried out in phosphate buffer saline. Group 3 mice were immunized with the plasmid DNAin non-ionic surfactant vesicles (NISV) and Group 1 was served as untreated control. The effect of immunization wasstudied in terms of anti-GnRH-I antibody response (OD value at A540 ± SD), suppression of ovarian folliculogenesis,altered uterine histoarchitecture and impaired fertility in vivo in mating trials. In study week 24 OD values of anti-GnRH-I antibody response were 0.982 ± 0.231 in Group 3 mice, followed by 0.783 ± 0.191 in Group 2 in comparisonwith no response in Group 1 controls (0.237 ± 0.147). Results of mating trials showed conception failure invaccinated mice; 51, 18 and 05 pups were seen in the uteri of Groups 1, 2 and 3 mice respectively. There wassignificant (p>0.001) reduction in the weight of ovaries in Group 2 (8.50 ± 2.38 mg) and Group 3 (7.25 ± 0.95 mg)mice compared to Group 1 control (15.00 ± 1.41 mg). Significant reduction of ovarian folliculogenesis was seen inGroup 2 (p>0.001) and Group 3 mice (p>0.01). In conclusion, the plasmid DNA vaccine delivered in female micewith HVJE and NISV induced significantly (p>0.001) higher levels of anti-GnRH-I antibody response, suppressedovarian and uterine function and impaired fertility in vivo.

AB - Induction of an appropriate immune response against gonadotrophin releasing hormone (GnRH-I) disrupt fertility,reduce fecundity and regress tumours of reproductive system.To disrupt fertility a plasmid DNA vaccine wasengineered coding eight repeats of GnRH-I and eight T-helper epitopes. Translation efficiency of the vaccine wasevaluated in undifferentiated COS1 cells and found to release GnRH-I fusion protein in culture supernatant. Swissalbino female mice (N=24) were immunized with 50μg plasmid DNA construct in study weeks 0, 3, 6, 9 and 12.Group 2 mice were primed with the plasmid DNA in hemagglutinating virus of japanese envelope (HVJE) vector andsubsequent boosts were carried out in phosphate buffer saline. Group 3 mice were immunized with the plasmid DNAin non-ionic surfactant vesicles (NISV) and Group 1 was served as untreated control. The effect of immunization wasstudied in terms of anti-GnRH-I antibody response (OD value at A540 ± SD), suppression of ovarian folliculogenesis,altered uterine histoarchitecture and impaired fertility in vivo in mating trials. In study week 24 OD values of anti-GnRH-I antibody response were 0.982 ± 0.231 in Group 3 mice, followed by 0.783 ± 0.191 in Group 2 in comparisonwith no response in Group 1 controls (0.237 ± 0.147). Results of mating trials showed conception failure invaccinated mice; 51, 18 and 05 pups were seen in the uteri of Groups 1, 2 and 3 mice respectively. There wassignificant (p>0.001) reduction in the weight of ovaries in Group 2 (8.50 ± 2.38 mg) and Group 3 (7.25 ± 0.95 mg)mice compared to Group 1 control (15.00 ± 1.41 mg). Significant reduction of ovarian folliculogenesis was seen inGroup 2 (p>0.001) and Group 3 mice (p>0.01). In conclusion, the plasmid DNA vaccine delivered in female micewith HVJE and NISV induced significantly (p>0.001) higher levels of anti-GnRH-I antibody response, suppressedovarian and uterine function and impaired fertility in vivo.

KW - mice

KW - DNA

KW - vaccine

KW - GnRH-I

KW - in vivo fertility

KW - ovarian folliculogenesis

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DO - 10.4172/2157-7560.1000282

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