Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy

V.A. Ferro, R. Costa, K.C. Carter, M.J. Harvey, M.M. Waterston, A. Mullen, C. Matschke, J.F.S. Mann, A. Colston, W.H. Stimson

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid and formulated with different adjuvants (non-ionic surfactant vesicles, aluminium hydroxide, Quil A, PLGA (poly(lactide-co-glycolide)/triacetin), and Quil A/PLGA). A comparison of the anti-fertility efficacy of the formulations was made by examining specific antibody levels, antibody subclasses, endocrine ablation and gonadal atrophy. The production of IgG2b antibody provided the best correlation for castration. PLGA was considered the most effective adjuvant as it produced a consistent anti-fertility response in all the treated animals.
LanguageEnglish
Pages1024-31
Number of pages8
JournalVaccine
Volume22
Issue number8
DOIs
Publication statusPublished - 25 Feb 2004

Fingerprint

Gonadotropin-Releasing Hormone
adjuvants
Fertility
Vaccines
triacetin
immune response
vaccines
antigens
aluminum hydroxide
antibodies
toxoids
tetanus
Triacetin
antibody formation
castration
atrophy
Aluminum Hydroxide
Polyglactin 910
Tetanus Toxoid
Antibodies

Keywords

  • GnRH
  • anti-fertility
  • vaccine

Cite this

Ferro, V.A. ; Costa, R. ; Carter, K.C. ; Harvey, M.J. ; Waterston, M.M. ; Mullen, A. ; Matschke, C. ; Mann, J.F.S. ; Colston, A. ; Stimson, W.H. / Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy. In: Vaccine. 2004 ; Vol. 22, No. 8. pp. 1024-31.
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Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy. / Ferro, V.A.; Costa, R.; Carter, K.C.; Harvey, M.J.; Waterston, M.M.; Mullen, A.; Matschke, C.; Mann, J.F.S.; Colston, A.; Stimson, W.H.

In: Vaccine, Vol. 22, No. 8, 25.02.2004, p. 1024-31.

Research output: Contribution to journalArticle

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T1 - Immune responses to a GnRH-based anti-fertility immunogen, induced by different adjuvants and subsequent effect on vaccine efficacy

AU - Ferro, V.A.

AU - Costa, R.

AU - Carter, K.C.

AU - Harvey, M.J.

AU - Waterston, M.M.

AU - Mullen, A.

AU - Matschke, C.

AU - Mann, J.F.S.

AU - Colston, A.

AU - Stimson, W.H.

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AB - A modified GnRH peptide (CHWSYGLRPG-NH2) was conjugated to tetanus toxoid and formulated with different adjuvants (non-ionic surfactant vesicles, aluminium hydroxide, Quil A, PLGA (poly(lactide-co-glycolide)/triacetin), and Quil A/PLGA). A comparison of the anti-fertility efficacy of the formulations was made by examining specific antibody levels, antibody subclasses, endocrine ablation and gonadal atrophy. The production of IgG2b antibody provided the best correlation for castration. PLGA was considered the most effective adjuvant as it produced a consistent anti-fertility response in all the treated animals.

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KW - anti-fertility

KW - vaccine

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