Immune adjuvant effect of V. cholerae O1 derived Proteoliposome coadministered by intranasal route with Vi polysaccharide from Salmonella Typhi

Reinaldo Acevedo, Adriana Callico, Yisabel Arunguren, Caridad Zayas, Yolanda Valdes, Oliver Perez, Valerie Ferro, Jose L Perez

Research output: Contribution to journalSpecial issue

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Abstract

Proteoliposome derived from Vibrio cholerae O1 (PLc) is a nanoscaled structure obtained by detergent extraction process. Intranasal (i.n) administration of PLc was immunogenic at mucosal and systemic level vs. V. cholerae; however the adjuvant potential of this structure for non-cholera antigens has not been proven yet. The aim of this work was to evaluate the effect of coadministering PLc with the Vi polysaccharide antigen (Poli Vi) of S. Typhi by i.n route. The results showed that Poli Vi coadministered with PLc (PLc+Poli Vi) induce higher IgA response in saliva (p<0.01) and faeces (p<0.01) than Poli Vi administered alone. Likewise, the IgG response in sera was higher in animals immunised with PLc+Poli Vi (p<0.01). Furthermore, IgG induced in sera of mice immunised with PLc+Poli Vi was similar (p>0.05) to that induced in a group of mice immunised by parenteral route with the Cuban anti-typhoid vaccine vax-TyVi®, although this vaccine did not induce mucosal response. In conclusion, this work demonstrates that PLc can be used as mucosal adjuvant to potentiate the immune response against a polysaccharide antigen like Poli Vi.
Original languageEnglish
Article numberS10
JournalBMC Immunology
Volume14
Issue numberSupplement 1
DOIs
Publication statusPublished - 25 Feb 2013
EventVaccipharma 2012 - , Cuba
Duration: 15 Jun 201221 Jun 2012

Keywords

  • Salmonella Typhi
  • non-cholera antigens
  • Vi polysaccharide antigen
  • immunisation

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