Abstract
| Original language | English |
|---|---|
| Article number | S10 |
| Journal | BMC Immunology |
| Volume | 14 |
| Issue number | Supplement 1 |
| DOIs | |
| Publication status | Published - 25 Feb 2013 |
| Event | Vaccipharma 2012 - , Cuba Duration: 15 Jun 2012 → 21 Jun 2012 |
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Keywords
- Salmonella Typhi
- non-cholera antigens
- Vi polysaccharide antigen
- immunisation
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Immune adjuvant effect of V. cholerae O1 derived Proteoliposome coadministered by intranasal route with Vi polysaccharide from Salmonella Typhi. / Acevedo, Reinaldo; Callico, Adriana; Arunguren, Yisabel; Zayas, Caridad; Valdes, Yolanda; Perez, Oliver; Ferro, Valerie; Perez, Jose L.
In: BMC Immunology, Vol. 14, No. Supplement 1, S10, 25.02.2013.Research output: Contribution to journal › Special issue
TY - JOUR
T1 - Immune adjuvant effect of V. cholerae O1 derived Proteoliposome coadministered by intranasal route with Vi polysaccharide from Salmonella Typhi
AU - Acevedo, Reinaldo
AU - Callico, Adriana
AU - Arunguren, Yisabel
AU - Zayas, Caridad
AU - Valdes, Yolanda
AU - Perez, Oliver
AU - Ferro, Valerie
AU - Perez, Jose L
PY - 2013/2/25
Y1 - 2013/2/25
N2 - Proteoliposome derived from Vibrio cholerae O1 (PLc) is a nanoscaled structure obtained by detergent extraction process. Intranasal (i.n) administration of PLc was immunogenic at mucosal and systemic level vs. V. cholerae; however the adjuvant potential of this structure for non-cholera antigens has not been proven yet. The aim of this work was to evaluate the effect of coadministering PLc with the Vi polysaccharide antigen (Poli Vi) of S. Typhi by i.n route. The results showed that Poli Vi coadministered with PLc (PLc+Poli Vi) induce higher IgA response in saliva (p<0.01) and faeces (p<0.01) than Poli Vi administered alone. Likewise, the IgG response in sera was higher in animals immunised with PLc+Poli Vi (p<0.01). Furthermore, IgG induced in sera of mice immunised with PLc+Poli Vi was similar (p>0.05) to that induced in a group of mice immunised by parenteral route with the Cuban anti-typhoid vaccine vax-TyVi®, although this vaccine did not induce mucosal response. In conclusion, this work demonstrates that PLc can be used as mucosal adjuvant to potentiate the immune response against a polysaccharide antigen like Poli Vi.
AB - Proteoliposome derived from Vibrio cholerae O1 (PLc) is a nanoscaled structure obtained by detergent extraction process. Intranasal (i.n) administration of PLc was immunogenic at mucosal and systemic level vs. V. cholerae; however the adjuvant potential of this structure for non-cholera antigens has not been proven yet. The aim of this work was to evaluate the effect of coadministering PLc with the Vi polysaccharide antigen (Poli Vi) of S. Typhi by i.n route. The results showed that Poli Vi coadministered with PLc (PLc+Poli Vi) induce higher IgA response in saliva (p<0.01) and faeces (p<0.01) than Poli Vi administered alone. Likewise, the IgG response in sera was higher in animals immunised with PLc+Poli Vi (p<0.01). Furthermore, IgG induced in sera of mice immunised with PLc+Poli Vi was similar (p>0.05) to that induced in a group of mice immunised by parenteral route with the Cuban anti-typhoid vaccine vax-TyVi®, although this vaccine did not induce mucosal response. In conclusion, this work demonstrates that PLc can be used as mucosal adjuvant to potentiate the immune response against a polysaccharide antigen like Poli Vi.
KW - Salmonella Typhi
KW - non-cholera antigens
KW - Vi polysaccharide antigen
KW - immunisation
UR - http://www.biomedcentral.com/bmcimmunol/archive
U2 - 10.1186/1471-2172-14-S1-S10
DO - 10.1186/1471-2172-14-S1-S10
M3 - Special issue
VL - 14
JO - BMC Immunology
JF - BMC Immunology
SN - 1471-2172
IS - Supplement 1
M1 - S10
ER -