Imaging pericytes and the regulation of cerebral blood flow

Katie Boyd, Matthew Hammond-Haley, Rozan Vroman, Catherine N. Hall

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The brain's high energy requirements drive the need for close coupling of local neuronal activity to blood supply. Capillaries have been shown to dilate before arterioles in response to sensory stimulation, pointing to a key role for microvascular pericytes in mediating cerebrovascular dynamics. However, many aspects of these cells' function remain unknown and even controversial, from their identification, to the mechanism and regulation of their contractility in physiology and disease. Investigating how pericytes regulate vascular diameter is therefore likely to be the subject of many future experiments. Here we provide protocols for three different techniques (ex vivo slice imaging, in vivo imaging, and immunohistochemistry) that are highly valuable for performing such experiments.
Original languageEnglish
Title of host publicationPericytes
Subtitle of host publicationMethods and Protocols
EditorsBruno M. Péault
Place of PublicationCham, Switzerland
PublisherSpringer
Pages89-117
Number of pages29
ISBN (Electronic)9781071610565
ISBN (Print)9781071610589, 9781071610558
DOIs
Publication statusPublished - 13 Feb 2021

Publication series

NameMethods in Molecular Biology
Volume2235
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • imaging
  • neurovascular coupling
  • pericyte

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