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The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.
|Patent number||WO 2009/022125 A1|
|IPC||C12Q 1/68 (2006.1)|
|Publication status||Published - 19 Feb 2009|
- Raman spectroscopy
- nucleic acid sequences
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