Identification of nucleic acid sequences

Karen Faulds; Duncan Graham; William Smith; Alastair Ricketts

Identification of nucleic acid sequences

Karen Faulds (Inventor), Duncan Graham (Inventor), William Smith (Inventor), Alastair Ricketts (Inventor)

Pure And Applied Chemistry

Research output: Patent

Abstract

The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.

Original language	English
Patent number	WO 2009/022125 A1
IPC	C12Q 1/68 (2006.1)
Publication status	Published - 19 Feb 2009

Keywords

Raman spectroscopy
nucleic acid sequences
spectroscopy

1 Finished

EXOSERRS - AMPLIFICATION FREE DIRECT GENOMIC SEQUENCE ANALYSIS BY OPTICAL SPECTROSCOPY
Faulds, K.
EPSRC (Engineering and Physical Sciences Research Council)
1/10/07 → 31/10/11
Project: Research

Cite this

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title = "Identification of nucleic acid sequences",

abstract = "The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.",

keywords = "Raman spectroscopy , nucleic acid sequences, spectroscopy ",

author = "Karen Faulds and Duncan Graham and William Smith and Alastair Ricketts",

year = "2009",

month = feb,

day = "19",

language = "English",

type = "Patent",

note = "WO 2009/022125 A1; C12Q 1/68 (2006.1)",

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TY - PAT

T1 - Identification of nucleic acid sequences

AU - Faulds, Karen

AU - Graham, Duncan

AU - Smith, William

AU - Ricketts, Alastair

PY - 2009/2/19

Y1 - 2009/2/19

N2 - The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.

AB - The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.

KW - Raman spectroscopy

KW - nucleic acid sequences

KW - spectroscopy

M3 - Patent

M1 - WO 2009/022125 A1

ER -

Identification of nucleic acid sequences

Abstract

Keywords

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Projects

EXOSERRS - AMPLIFICATION FREE DIRECT GENOMIC SEQUENCE ANALYSIS BY OPTICAL SPECTROSCOPY

Cite this