Abstract
| Original language | English |
|---|---|
| Patent number | WO 2009/022125 A1 |
| IPC | C12Q 1/68 (2006.1) |
| Publication status | Published - 19 Feb 2009 |
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Keywords
- Raman spectroscopy
- nucleic acid sequences
- spectroscopy
Cite this
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Identification of nucleic acid sequences. / Faulds, Karen (Inventor); Graham, Duncan (Inventor); Smith, William (Inventor); Ricketts, Alastair (Inventor).
IPC No.: C12Q 1/68 (2006.1). Patent No.: WO 2009/022125 A1.Research output: Patent
TY - PAT
T1 - Identification of nucleic acid sequences
AU - Faulds, Karen
AU - Graham, Duncan
AU - Smith, William
AU - Ricketts, Alastair
PY - 2009/2/19
Y1 - 2009/2/19
N2 - The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.
AB - The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.
KW - Raman spectroscopy
KW - nucleic acid sequences
KW - spectroscopy
M3 - Patent
M1 - WO 2009/022125 A1
ER -