Identification of nucleic acid sequences

Karen Faulds (Inventor), Duncan Graham (Inventor), William Smith (Inventor), Alastair Ricketts (Inventor)

Research output: Patent

Abstract

The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.
Original languageEnglish
Patent numberWO 2009/022125 A1
IPCC12Q 1/68 (2006.1)
Publication statusPublished - 19 Feb 2009

Fingerprint

Nucleic acid sequences
Nucleic Acids
Nucleic Acid Probes
Labels
Exonucleases
Patents and inventions
Raman spectroscopy
Molecules

Keywords

  • Raman spectroscopy
  • nucleic acid sequences
  • spectroscopy

Cite this

Faulds, K., Graham, D., Smith, W., & Ricketts, A. (2009). IPC No. C12Q 1/68 (2006.1). Identification of nucleic acid sequences. (Patent No. WO 2009/022125 A1).
Faulds, Karen (Inventor) ; Graham, Duncan (Inventor) ; Smith, William (Inventor) ; Ricketts, Alastair (Inventor). / Identification of nucleic acid sequences. IPC No.: C12Q 1/68 (2006.1). Patent No.: WO 2009/022125 A1.
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title = "Identification of nucleic acid sequences",
abstract = "The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.",
keywords = "Raman spectroscopy , nucleic acid sequences, spectroscopy",
author = "Karen Faulds and Duncan Graham and William Smith and Alastair Ricketts",
year = "2009",
month = "2",
day = "19",
language = "English",
type = "Patent",
note = "WO 2009/022125 A1; C12Q 1/68 (2006.1)",

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Faulds, K, Graham, D, Smith, W & Ricketts, A 2009, Identification of nucleic acid sequences, Patent No. WO 2009/022125 A1, IPC No. C12Q 1/68 (2006.1).

Identification of nucleic acid sequences. / Faulds, Karen (Inventor); Graham, Duncan (Inventor); Smith, William (Inventor); Ricketts, Alastair (Inventor).

IPC No.: C12Q 1/68 (2006.1). Patent No.: WO 2009/022125 A1.

Research output: Patent

TY - PAT

T1 - Identification of nucleic acid sequences

AU - Faulds, Karen

AU - Graham, Duncan

AU - Smith, William

AU - Ricketts, Alastair

PY - 2009/2/19

Y1 - 2009/2/19

N2 - The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.

AB - The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex: and (ii) detecting the label by Raman spectroscopy.

KW - Raman spectroscopy

KW - nucleic acid sequences

KW - spectroscopy

M3 - Patent

M1 - WO 2009/022125 A1

ER -

Faulds K, Graham D, Smith W, Ricketts A, inventors. Identification of nucleic acid sequences. C12Q 1/68 (2006.1). 2009 Feb 19.