Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA

Carika  Weldon; Isabelle Behm-Ansmant; Laurence H Hurley; Glenn A Burley; Christiane  Branlant; Ian C Eperon; Cyril Dominguez

doi:10.1038/nchembio.2228

Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA

Carika Weldon, Isabelle Behm-Ansmant, Laurence H Hurley, Glenn A Burley, Christiane Branlant, Ian C Eperon, Cyril Dominguez

Pure And Applied Chemistry

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Abstract

RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.

Original language	English
Pages (from-to)	18-20
Number of pages	3
Journal	Nature Chemical Biology
Volume	13
Early online date	7 Nov 2016
DOIs	https://doi.org/10.1038/nchembio.2228
Publication status	E-pub ahead of print - 7 Nov 2016

Keywords

chemical tools
RNA
RNA splicing

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10.1038/nchembio.2228

Weldon-etal-NCB-2016-Identification-of-G-quadruplexes-in-long-functional-RNAsAccepted author manuscript, 487 KB

Glenn Burley
- glenn.burleystrath.acuk
- Pure And Applied Chemistry - Professor
Person: Academic

Cite this

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title = "Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA",

abstract = "RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.",

keywords = "chemical tools, RNA, RNA splicing",

author = "Carika Weldon and Isabelle Behm-Ansmant and Hurley, {Laurence H} and Burley, {Glenn A} and Christiane Branlant and Eperon, {Ian C} and Cyril Dominguez",

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T1 - Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA

AU - Weldon, Carika

AU - Behm-Ansmant, Isabelle

AU - Hurley, Laurence H

AU - Burley, Glenn A

AU - Branlant, Christiane

AU - Eperon, Ian C

AU - Dominguez, Cyril

PY - 2016/11/7

Y1 - 2016/11/7

N2 - RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.

AB - RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.

KW - chemical tools

KW - RNA

KW - RNA splicing

U2 - 10.1038/nchembio.2228

DO - 10.1038/nchembio.2228

M3 - Article

SN - 1552-4450

VL - 13

SP - 18

EP - 20

JO - Nature Chemical Biology

JF - Nature Chemical Biology

ER -

Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA

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