Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA

Carika Weldon, Isabelle Behm-Ansmant, Laurence H Hurley, Glenn A Burley, Christiane Branlant, Ian C Eperon, Cyril Dominguez

Research output: Contribution to journalArticle

27 Citations (Scopus)
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Abstract

RNA G-quadruplex (G4) structures are thought to affect biological processes, including translation and pre-mRNA splicing, but it is not possible at present to demonstrate that they form naturally at specific sequences in long functional RNA molecules. We developed a new strategy, footprinting of long 7-deazaguanine-substituted RNAs (FOLDeR), that allows the formation of G4s to be confirmed in long RNAs and under functional conditions.
Original languageEnglish
Pages (from-to)18-20
Number of pages3
JournalNature Chemical Biology
Volume13
Early online date7 Nov 2016
DOIs
Publication statusE-pub ahead of print - 7 Nov 2016

Keywords

  • chemical tools
  • RNA
  • RNA splicing

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  • Cite this

    Weldon, C., Behm-Ansmant, I., Hurley, L. H., Burley, G. A., Branlant, C., Eperon, I. C., & Dominguez, C. (2016). Identification of G-quadruplexes in long functional RNAs using 7-deazaguanine RNA. Nature Chemical Biology, 13, 18-20. https://doi.org/10.1038/nchembio.2228