TY - JOUR
T1 - Identification and characterisation of Staphylococcus aureus on low cost screen printed carbon electrodes using impedance spectroscopy
AU - Ward, A.C.
AU - Hannah, A.J.
AU - Kendrick, S.L.
AU - Tucker, N.P.
AU - MacGregor, G.
AU - Connolly, P.
PY - 2018/7/31
Y1 - 2018/7/31
N2 - Staphylococcus aureus infections are a cause of significant morbidity and mortality, in addition to representing a considerable economic burden. The aim of this study was to explore a low cost screen printed electrode as a sensor for the detection of S. aureus using impedance spectroscopy. S. aureus was incubated in chambers containing the electrodes and the results analysed using a novel normalisation approach. These results show that it is possible to detect the presence of S. aureus in LB media after 30 minutes incubation of a 1% growth culture, in addition to being able to see immediate cell concentration dependent changes in 0.9% NaCl. These observations imply that a number of electrochemical mechanisms cause a change in the impedance as a result of the presence of S. aureus, including adsorption to the electrode surface and the metabolism of the bacteria during growth. The study suggests that this detection approach would be useful in a number of clinical scenarios where S. aureus leads to difficult to treat infections.
AB - Staphylococcus aureus infections are a cause of significant morbidity and mortality, in addition to representing a considerable economic burden. The aim of this study was to explore a low cost screen printed electrode as a sensor for the detection of S. aureus using impedance spectroscopy. S. aureus was incubated in chambers containing the electrodes and the results analysed using a novel normalisation approach. These results show that it is possible to detect the presence of S. aureus in LB media after 30 minutes incubation of a 1% growth culture, in addition to being able to see immediate cell concentration dependent changes in 0.9% NaCl. These observations imply that a number of electrochemical mechanisms cause a change in the impedance as a result of the presence of S. aureus, including adsorption to the electrode surface and the metabolism of the bacteria during growth. The study suggests that this detection approach would be useful in a number of clinical scenarios where S. aureus leads to difficult to treat infections.
KW - screen printing
KW - impedance spectroscopy
KW - low cost diagnostics
KW - Staphylococcus aureus
KW - rapid testing
KW - bacteria
UR - https://www.sciencedirect.com/journal/biosensors-and-bioelectronics
U2 - 10.1016/j.bios.2018.03.048
DO - 10.1016/j.bios.2018.03.048
M3 - Article
VL - 110
SP - 65
EP - 70
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
SN - 0956-5663
ER -