Hydrolysis of growth defined plant biomass using enzyme cocktails: an optimisation approach for enhanced fermentable hydrolysates

Henry P. Oamen, Ebenezer O. Ojo, Phil J. Hobbs, Andy Retter

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Abstract

In this experiment, optimum experimental conditions for the enzymatic hydrolysis of grass at different stages of growth were obtained by using the Taguchi methodology. Rye grass silage and three growth stages of Italian rye grass samples were used to determine optimum hydrolysis conditions. Five factors (pretreatment, enzyme composition, incubation temperature, pretreatment time, pH) influencing the hydrolysis process were studied at the individual and interactive levels. All selected experimental factors influenced the hydrolysis of grass. At the individual level, pretreatment of grass with NaOH and enzyme composition had the greatest influence (75% and 14.7% of the variance respectively) on enzymatic hydrolysis. Incubation temperature, pretreatment time and pH had influences of 8.1%, 2.2% and 0.055%, respectively. pH and incubation temperature had the most significant interaction effect (65.6%) on enzymatic hydrolysis. The factors with the least individual influence had the most significant interaction effect on enzymatic hydrolysis. Hydrolysis was improved when optimised conditions were applied to different growth stages of Italian rye grass.
Original languageEnglish
Article numbere00077
Number of pages9
JournalScientific African
Volume3
Early online date30 Apr 2019
DOIs
Publication statusPublished - 30 May 2019

Keywords

  • enzymatic hydrolysis
  • Lignocellulose
  • Taguchi methodology
  • pretreatment
  • grass
  • rye grass silage
  • hydrolysis conditions

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