Hydrogels for the detection and management of protease levels

Alison G. Patrick, Rein V. Ulijn

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The design of hydrogels that simultaneously report protease activity and remove excess protease from solution is elucidated. The hydrogels, based on amino-PEGA, combine enzyme-specific peptides flanked with FRET complimented by charged amino acid residues that facilitate protease uptake via short range electrostatic interactions. Enzymatic response was analysed using a combination of fluorescence spectroscopy, two-photon microscopy and UV/Vis spectroscopy. An optimised elastase-responsive hydrogel resulted in lowering of elastase levels below those typical of chronic wounds. The versatility of the modular-design approach was demonstrated by development of matrix metalloprotease and chymotrypsin sensitive systems.

Original languageEnglish
Pages (from-to)1184-1193
Number of pages10
JournalMacromolecular Bioscience
Volume10
Issue number10
Early online date30 Jun 2010
DOIs
Publication statusPublished - 8 Oct 2010

Keywords

  • detection and management
  • protease levels
  • hydrogels
  • peptides
  • sensors
  • enzymes
  • fluorescence

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