Hydrogels for the detection and management of protease levels

Alison G. Patrick, Rein V. Ulijn

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The design of hydrogels that simultaneously report protease activity and remove excess protease from solution is elucidated. The hydrogels, based on amino-PEGA, combine enzyme-specific peptides flanked with FRET complimented by charged amino acid residues that facilitate protease uptake via short range electrostatic interactions. Enzymatic response was analysed using a combination of fluorescence spectroscopy, two-photon microscopy and UV/Vis spectroscopy. An optimised elastase-responsive hydrogel resulted in lowering of elastase levels below those typical of chronic wounds. The versatility of the modular-design approach was demonstrated by development of matrix metalloprotease and chymotrypsin sensitive systems.

Original languageEnglish
Pages (from-to)1184-1193
Number of pages10
JournalMacromolecular Bioscience
Volume10
Issue number10
Early online date30 Jun 2010
DOIs
Publication statusPublished - 8 Oct 2010

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Hydrogels
Peptide Hydrolases
Pancreatic Elastase
Hydrogel
Fluorescence Spectrometry
Fluorescence spectroscopy
Chymotrypsin
Metalloproteases
Coulomb interactions
Ultraviolet spectroscopy
Static Electricity
Photons
Peptides
Amino acids
Microscopy
Spectrum Analysis
Microscopic examination
Enzymes
Amino Acids
Wounds and Injuries

Keywords

  • detection and management
  • protease levels
  • hydrogels
  • peptides
  • sensors
  • enzymes
  • fluorescence

Cite this

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Hydrogels for the detection and management of protease levels. / Patrick, Alison G.; Ulijn, Rein V.

In: Macromolecular Bioscience, Vol. 10, No. 10, 08.10.2010, p. 1184-1193.

Research output: Contribution to journalArticle

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