Human natural killer (NK) cell activation by luteolin from Brucea javanica leaves

Norzila Ismail; Hasmah Abdullah; Veronique Seidel; Dino Rotondo

doi:10.5897/JCREO2012.012

Human natural killer (NK) cell activation by luteolin from Brucea javanica leaves

Norzila Ismail, Hasmah Abdullah, Veronique Seidel, Dino Rotondo

Strathclyde Institute Of Pharmacy And Biomedical Sciences

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Abstract

There is growing interest in using natural substances from plants to potentially enhance anticancer activity. A key target in this respect is natural killer (NK) cells in order to generate an anticancer-immune response. Luteolin used in this study was derived from a local plant Brucea javanica leaves extract; through a bioassay-guided fractionation. The expression of interleukin-2 (IL-2) and interferon-γ (IFN-γ) was increased after 20 hours treatment of 0.1μM luteolin in human mixed lymphocytes, which reflects the activation of NK cells. This was further confirmed in co-culture experiments. NK cell-induced K562 target cell death was increased in the presence of luteolin. These results show that luteolin activates NK cells to kill target cells indicating the potential use of luteolin as
an anticancer immunostimulant.

Original language	English
Article number	B3D352E59611
Pages (from-to)	10-14
Number of pages	5
Journal	Journal of Cancer Research and Experimental Oncology
Volume	10
Issue number	2
DOIs	https://doi.org/10.5897/JCREO2012.012
Publication status	Published - 31 Dec 2018

Keywords

Luteolin
Brucea javanica
natural killer cells
interleukin-2
interferon-γ
cancer cell killing

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.5897/JCREO2012.012Licence: CC BY 4.0

Ismail-etal-JCREO-2018-Human-natural-killer-NK-cell-activation-by-luteolin-from-Brucea-javanica-leavesFinal published version, 266 KBLicence: CC BY 4.0

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title = "Human natural killer (NK) cell activation by luteolin from Brucea javanica leaves",

abstract = "There is growing interest in using natural substances from plants to potentially enhance anticancer activity. A key target in this respect is natural killer (NK) cells in order to generate an anticancer-immune response. Luteolin used in this study was derived from a local plant Brucea javanica leaves extract; through a bioassay-guided fractionation. The expression of interleukin-2 (IL-2) and interferon-γ (IFN-γ) was increased after 20 hours treatment of 0.1μM luteolin in human mixed lymphocytes, which reflects the activation of NK cells. This was further confirmed in co-culture experiments. NK cell-induced K562 target cell death was increased in the presence of luteolin. These results show that luteolin activates NK cells to kill target cells indicating the potential use of luteolin as an anticancer immunostimulant. ",

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author = "Norzila Ismail and Hasmah Abdullah and Veronique Seidel and Dino Rotondo",

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AU - Ismail, Norzila

AU - Abdullah, Hasmah

AU - Seidel, Veronique

AU - Rotondo, Dino

PY - 2018/12/31

Y1 - 2018/12/31

N2 - There is growing interest in using natural substances from plants to potentially enhance anticancer activity. A key target in this respect is natural killer (NK) cells in order to generate an anticancer-immune response. Luteolin used in this study was derived from a local plant Brucea javanica leaves extract; through a bioassay-guided fractionation. The expression of interleukin-2 (IL-2) and interferon-γ (IFN-γ) was increased after 20 hours treatment of 0.1μM luteolin in human mixed lymphocytes, which reflects the activation of NK cells. This was further confirmed in co-culture experiments. NK cell-induced K562 target cell death was increased in the presence of luteolin. These results show that luteolin activates NK cells to kill target cells indicating the potential use of luteolin as an anticancer immunostimulant.

AB - There is growing interest in using natural substances from plants to potentially enhance anticancer activity. A key target in this respect is natural killer (NK) cells in order to generate an anticancer-immune response. Luteolin used in this study was derived from a local plant Brucea javanica leaves extract; through a bioassay-guided fractionation. The expression of interleukin-2 (IL-2) and interferon-γ (IFN-γ) was increased after 20 hours treatment of 0.1μM luteolin in human mixed lymphocytes, which reflects the activation of NK cells. This was further confirmed in co-culture experiments. NK cell-induced K562 target cell death was increased in the presence of luteolin. These results show that luteolin activates NK cells to kill target cells indicating the potential use of luteolin as an anticancer immunostimulant.

KW - Luteolin

KW - Brucea javanica

KW - natural killer cells

KW - interleukin-2

KW - interferon-γ

KW - cancer cell killing

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Human natural killer (NK) cell activation by luteolin from Brucea javanica leaves

Abstract

Keywords

UN SDGs

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