HPLC imprinted-stationary phase prepared by precipitation polymerisation for the determination of thiabendazole in fruit

E. Turiel, J. L. Tadeo, P. A. G. Cormack, A. Martin-Esteban

Research output: Contribution to journalArticle

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Abstract

A molecularly imprinted polymer (MIP) tailored for the HPLC determination of the fungicide thiabendazole (TBZ) has been synthesised in one single preparative step by precipitation polymerisation in an acetonitrile/toluene co-solvent, using TBZ as template molecule, methacrylic acid as functional monomer and divinylbenzene-80 as crosslinker. The imprinted polymer particulates obtained were characterised by scanning electron microscopy and nitrogen sorption porosimetry. These analyses showed clearly that spherical polymer particulates (polymer microspheres) with narrow size distributions (average particle diameter ∼3.5 µm) and well-developed pore structures had been produced. The imprinted microspheres were packed into a stainless steel HPLC column (50 × 4.6 mm id) and evaluated as an imprinted stationary phase. The imprinting effect was demonstrated clearly, i.e., the column was observed to bind TBZ selectively, and the effect of different chromatographic parameters (e.g., temperature, flow-rate and elution solvents) on TBZ retention/elution studied. Under optimised conditions, the TBZ-imprinted column was used for the HPLC-fluorescence (HPLC-F) determination of TBZ directly from orange (both whole fruit and juice), lemon, grape and strawberry extracts at low concentration levels in less than 15 min, without any need for a clean-up step in the analytical protocol.
LanguageEnglish
Pages1601-1607
Number of pages7
JournalAnalyst
Volume130
DOIs
Publication statusE-pub ahead of print - 14 Oct 2005

Fingerprint

Thiabendazole
Fruits
polymerization
Polymerization
Fruit
polymer
fruit
High Pressure Liquid Chromatography
Polymers
Microspheres
divinyl benzene
Fungicides
imprinting
fungicide
Pore structure
Acetonitrile
Particle size analysis
toluene
Toluene
Sorption

Cite this

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title = "HPLC imprinted-stationary phase prepared by precipitation polymerisation for the determination of thiabendazole in fruit",
abstract = "A molecularly imprinted polymer (MIP) tailored for the HPLC determination of the fungicide thiabendazole (TBZ) has been synthesised in one single preparative step by precipitation polymerisation in an acetonitrile/toluene co-solvent, using TBZ as template molecule, methacrylic acid as functional monomer and divinylbenzene-80 as crosslinker. The imprinted polymer particulates obtained were characterised by scanning electron microscopy and nitrogen sorption porosimetry. These analyses showed clearly that spherical polymer particulates (polymer microspheres) with narrow size distributions (average particle diameter ∼3.5 µm) and well-developed pore structures had been produced. The imprinted microspheres were packed into a stainless steel HPLC column (50 × 4.6 mm id) and evaluated as an imprinted stationary phase. The imprinting effect was demonstrated clearly, i.e., the column was observed to bind TBZ selectively, and the effect of different chromatographic parameters (e.g., temperature, flow-rate and elution solvents) on TBZ retention/elution studied. Under optimised conditions, the TBZ-imprinted column was used for the HPLC-fluorescence (HPLC-F) determination of TBZ directly from orange (both whole fruit and juice), lemon, grape and strawberry extracts at low concentration levels in less than 15 min, without any need for a clean-up step in the analytical protocol.",
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HPLC imprinted-stationary phase prepared by precipitation polymerisation for the determination of thiabendazole in fruit. / Turiel, E.; Tadeo, J. L.; Cormack, P. A. G.; Martin-Esteban, A.

In: Analyst, Vol. 130, 14.10.2005, p. 1601-1607.

Research output: Contribution to journalArticle

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AB - A molecularly imprinted polymer (MIP) tailored for the HPLC determination of the fungicide thiabendazole (TBZ) has been synthesised in one single preparative step by precipitation polymerisation in an acetonitrile/toluene co-solvent, using TBZ as template molecule, methacrylic acid as functional monomer and divinylbenzene-80 as crosslinker. The imprinted polymer particulates obtained were characterised by scanning electron microscopy and nitrogen sorption porosimetry. These analyses showed clearly that spherical polymer particulates (polymer microspheres) with narrow size distributions (average particle diameter ∼3.5 µm) and well-developed pore structures had been produced. The imprinted microspheres were packed into a stainless steel HPLC column (50 × 4.6 mm id) and evaluated as an imprinted stationary phase. The imprinting effect was demonstrated clearly, i.e., the column was observed to bind TBZ selectively, and the effect of different chromatographic parameters (e.g., temperature, flow-rate and elution solvents) on TBZ retention/elution studied. Under optimised conditions, the TBZ-imprinted column was used for the HPLC-fluorescence (HPLC-F) determination of TBZ directly from orange (both whole fruit and juice), lemon, grape and strawberry extracts at low concentration levels in less than 15 min, without any need for a clean-up step in the analytical protocol.

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