How to switch a fluorophore: from undesired blinking to controlled photoswitching

Research output: Contribution to journalReview article

79 Citations (Scopus)

Abstract

Molecular optical photoswitches based on fluorescent proteins and organic dyes are fundamental for super-resolution fluorescence imaging and tracking methods. Precise control of switching, bio-labeling compatibility, and high brightness make photoswitches broadly applicable. This review emphasizes the design and development of photoswitches and the requirements they need to fulfill for their successful application in single-molecule localization microscopy. Furthermore, we discuss recent developments in improving the photoswitching performance with a special focus on organic dyes.
LanguageEnglish
Pages1076-1087
Number of pages12
JournalChemical Society Reviews
Volume43
Issue number4
Early online date13 Aug 2013
DOIs
Publication statusPublished - 21 Feb 2014
Externally publishedYes

Fingerprint

blinking
Fluorophores
Coloring Agents
Dyes
dyes
Switches
Labeling
compatibility
marking
Luminance
Microscopic examination
brightness
Fluorescence
microscopy
proteins
Proteins
Imaging techniques
fluorescence
requirements
Molecules

Keywords

  • molecular optical photoswitches
  • organic dyes
  • super-resolution fluorescence imaging

Cite this

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How to switch a fluorophore : from undesired blinking to controlled photoswitching. / van de Linde, Sebastian; Sauer, Markus.

In: Chemical Society Reviews, Vol. 43, No. 4, 21.02.2014, p. 1076-1087.

Research output: Contribution to journalReview article

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