Hofmeister effects on activity and stability of alkaline phosphatase

Z. Yang, P.J. Halling

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have studied the effects on alkaline phosphatase of adding high concentrations (normally 1.0 M) of simple salts. It is necessary to allow for significant effects of salts on the extinction coefficient of the reaction product, and on the apparent pH of the buffer. Both activity and stability of the enzyme correlate well with the Hofmeister series in terms of the salt's kosmotropic/chaotropic properties, which are assessed by the Jones-Dole viscosity B coefficients (B+ for cations and B_ for anions). The catalytic activity or V-max/K-m of the enzyme showed a bell-shaped relationship with the (B_ -B+) values of the salts present, being optimal with salts (such as NaCl, KCl, and KNO3) where the anion and cation have similar kosmotropic/chaotropic properties. This effect is believed to be enzyme-specific and relates to the impact of both cations and anions on the enzyme's surface pH, active site, and catalytic mechanism. Anions play a more predominant role than cations in affecting enzyme stability. The rate of irreversible thermal inactivation is strongly reduced by addition of kosmotropic anions like SO42- (half-life increased from 8 to 580 min at 60 degrees C). This effect is general and the mechanism probably involves the ability of the ions to affect the water solvation layer around the enzyme molecule and to interact with both the surface and internal structure of the enzyme.
LanguageEnglish
Pages821-828
Number of pages7
JournalBBA - Biomembranes
Volume1804
Issue number4
DOIs
Publication statusPublished - Apr 2010

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Anions
Alkaline Phosphatase
Salts
Cations
Enzymes
Enzyme Stability
Viscosity
Half-Life
Catalytic Domain
Buffers
Solvation
Hot Temperature
Reaction products
Ions
Catalyst activity
Water
Molecules

Keywords

  • alkaline phosphatase
  • activity and stability
  • Hofmeister series
  • kosmotropicity
  • chaotropicity
  • jones–dole viscosity B coefficient

Cite this

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Hofmeister effects on activity and stability of alkaline phosphatase. / Yang, Z.; Halling, P.J.

In: BBA - Biomembranes, Vol. 1804, No. 4, 04.2010, p. 821-828.

Research output: Contribution to journalArticle

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