HOBS methods for enhancing resolution and sensitivity in small DNA oligonucleotide NMR studies

TY - JOUR

T1 - HOBS methods for enhancing resolution and sensitivity in small DNA oligonucleotide NMR studies

AU - McKenna, Josiah M.

AU - Parkinson, John A.

N1 - This is the accepted version of the following article: "HOBS methods for enhancing resolution and sensitivity in small DNA oligonucleotide NMR studies", McKenna, J. M. & Parkinson, J. 2014 In : Magnetic Resonance in Chemistry, which has been published in final form at http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-458Xa. In addition, authors may also transmit, print and share copies with colleagues, provided that there is no systematic distribution of the submitted version, e.g. posting on a listserve, network or automated delivery.

PY - 2015/4

Y1 - 2015/4

N2 - 1H NMR spectra from biopolymers give chemical shifts classified according to proton type and often suffer from signal degeneracy. Data from nucleic acids are particularly prone to this failing. Recent developments in proton broad-band decoupling techniques with the promise of enhanced resolution at full sensitivity have allowed us to investigate the application of homo-nuclear band-selective (HOBS) decoupling to the study of small synthetic DNA molecules and to compare these with results from classical and Pure Shift techniques. Improved signal resolution at full sensitivity in both HOBS-1D 1H and HOBS-2D [1H, 1H] NOESY NMR data are reported for three example small DNA molecules. Comparisons of 1H T1 and integrals of signals from HOBS-1D 1H and HOBS-2D [1H, 1H] NOESY NMR data with those of standard data collection methods are also reported. The results show that homo-nuclear HOBS-NOESY data are useful for data assignment purposes and have some merit for quantification purposes. In general we show that resolution and sensitivity enhancement of 1H NMR data for small DNA samples may be achieved without recourse to higher magnetic field strengths at full sensitivity in a band-selected manner.

AB - 1H NMR spectra from biopolymers give chemical shifts classified according to proton type and often suffer from signal degeneracy. Data from nucleic acids are particularly prone to this failing. Recent developments in proton broad-band decoupling techniques with the promise of enhanced resolution at full sensitivity have allowed us to investigate the application of homo-nuclear band-selective (HOBS) decoupling to the study of small synthetic DNA molecules and to compare these with results from classical and Pure Shift techniques. Improved signal resolution at full sensitivity in both HOBS-1D 1H and HOBS-2D [1H, 1H] NOESY NMR data are reported for three example small DNA molecules. Comparisons of 1H T1 and integrals of signals from HOBS-1D 1H and HOBS-2D [1H, 1H] NOESY NMR data with those of standard data collection methods are also reported. The results show that homo-nuclear HOBS-NOESY data are useful for data assignment purposes and have some merit for quantification purposes. In general we show that resolution and sensitivity enhancement of 1H NMR data for small DNA samples may be achieved without recourse to higher magnetic field strengths at full sensitivity in a band-selected manner.

KW - NMR

KW - 1H

KW - band-selective homo-nuclear decoupling

KW - HOBS

KW - NOESY

KW - T1

UR - http://onlinelibrary.wiley.com/journal/10.1002/(ISSN)1097-458Xa

U2 - 10.1002/mrc.4182

DO - 10.1002/mrc.4182

M3 - Article

VL - 53

SP - 249

EP - 255

JO - Magnetic Resonance in Chemistry

T2 - Magnetic Resonance in Chemistry

JF - Magnetic Resonance in Chemistry

SN - 0749-1581

IS - 4

ER -