Highly sensitive fluorescence detection on a biochip

T. Ruckstuhl , R. Blue, S. Spillman , N. Kent, H. McEvoy, S. Laib, C McDonagh , B. D. MacCraith

Research output: Contribution to conferenceAbstract

Abstract

A novel, generic lab-on-a-chip platform and associated readout instrumentation is presented. This high-sensitivity system has been developed for the efficient detection of surface-generated fluorescence in biomedical diagnostic applications. The proofof-principle polymer chip contains a 3×3 array of paraboloid elements designed to capture supercritical angle fluorescence (SAF) emitted from biorecognition zones on the top of each paraboloid. Each such element exhibits a fluorescence collection efficiency of 32%, comparable only to sophisticated microscope objectives of high numerical aperture. Furthermore, the chip optical design results in strict confinement of the fluorescence excitation to the surface. Consequently, this inexpensive chip combines the collecting power of modern microscopy optics with strong discrimination between surface and bulk fluorescence. The chip allows for simultaneous detection of 9 different targets (e.g. biomarkers) with very high sensitivity and also facilitates monitoring of receptor-ligand binding in real time. The chip is fabricated in the low fluorescence polymer Zeonor and is compatible with mass production via micro-injection moulding. The performance of the platform is demonstrated by application to a standard bioassay.

Conference

ConferencePushing the technology envelope III: The next generations of diagnostic testing, Oak Ridge Conference, San Jose, April 2006.
CountryUnited States
CitySan Jose
Period20/04/06 → …

Fingerprint

Biochips
Fluorescence
Polymers
Lab-on-a-chip
Optical design
Bioassay
Biomarkers
Injection molding
Optics
Microscopic examination
Microscopes
Ligands
Monitoring

Keywords

  • highly sensitive
  • fluorescence detection
  • biochip

Cite this

Ruckstuhl , T., Blue, R., Spillman , S., Kent, N., McEvoy, H., Laib, S., ... MacCraith , B. D. (2006). Highly sensitive fluorescence detection on a biochip. Abstract from Pushing the technology envelope III: The next generations of diagnostic testing, Oak Ridge Conference, San Jose, April 2006., San Jose, United States.
Ruckstuhl , T. ; Blue, R. ; Spillman , S. ; Kent, N. ; McEvoy, H. ; Laib, S. ; McDonagh , C ; MacCraith , B. D. / Highly sensitive fluorescence detection on a biochip. Abstract from Pushing the technology envelope III: The next generations of diagnostic testing, Oak Ridge Conference, San Jose, April 2006., San Jose, United States.
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title = "Highly sensitive fluorescence detection on a biochip",
abstract = "A novel, generic lab-on-a-chip platform and associated readout instrumentation is presented. This high-sensitivity system has been developed for the efficient detection of surface-generated fluorescence in biomedical diagnostic applications. The proofof-principle polymer chip contains a 3×3 array of paraboloid elements designed to capture supercritical angle fluorescence (SAF) emitted from biorecognition zones on the top of each paraboloid. Each such element exhibits a fluorescence collection efficiency of 32{\%}, comparable only to sophisticated microscope objectives of high numerical aperture. Furthermore, the chip optical design results in strict confinement of the fluorescence excitation to the surface. Consequently, this inexpensive chip combines the collecting power of modern microscopy optics with strong discrimination between surface and bulk fluorescence. The chip allows for simultaneous detection of 9 different targets (e.g. biomarkers) with very high sensitivity and also facilitates monitoring of receptor-ligand binding in real time. The chip is fabricated in the low fluorescence polymer Zeonor and is compatible with mass production via micro-injection moulding. The performance of the platform is demonstrated by application to a standard bioassay.",
keywords = "highly sensitive, fluorescence detection, biochip",
author = "T. Ruckstuhl and R. Blue and S. Spillman and N. Kent and H. McEvoy and S. Laib and C McDonagh and MacCraith, {B. D.}",
year = "2006",
language = "English",
note = "Pushing the technology envelope III: The next generations of diagnostic testing, Oak Ridge Conference, San Jose, April 2006. ; Conference date: 20-04-2006",

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Ruckstuhl , T, Blue, R, Spillman , S, Kent, N, McEvoy, H, Laib, S, McDonagh , C & MacCraith , BD 2006, 'Highly sensitive fluorescence detection on a biochip' Pushing the technology envelope III: The next generations of diagnostic testing, Oak Ridge Conference, San Jose, April 2006., San Jose, United States, 20/04/06, .

Highly sensitive fluorescence detection on a biochip. / Ruckstuhl , T. ; Blue, R.; Spillman , S.; Kent, N. ; McEvoy, H. ; Laib, S.; McDonagh , C; MacCraith , B. D.

2006. Abstract from Pushing the technology envelope III: The next generations of diagnostic testing, Oak Ridge Conference, San Jose, April 2006., San Jose, United States.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - Highly sensitive fluorescence detection on a biochip

AU - Ruckstuhl , T.

AU - Blue, R.

AU - Spillman , S.

AU - Kent, N.

AU - McEvoy, H.

AU - Laib, S.

AU - McDonagh , C

AU - MacCraith , B. D.

PY - 2006

Y1 - 2006

N2 - A novel, generic lab-on-a-chip platform and associated readout instrumentation is presented. This high-sensitivity system has been developed for the efficient detection of surface-generated fluorescence in biomedical diagnostic applications. The proofof-principle polymer chip contains a 3×3 array of paraboloid elements designed to capture supercritical angle fluorescence (SAF) emitted from biorecognition zones on the top of each paraboloid. Each such element exhibits a fluorescence collection efficiency of 32%, comparable only to sophisticated microscope objectives of high numerical aperture. Furthermore, the chip optical design results in strict confinement of the fluorescence excitation to the surface. Consequently, this inexpensive chip combines the collecting power of modern microscopy optics with strong discrimination between surface and bulk fluorescence. The chip allows for simultaneous detection of 9 different targets (e.g. biomarkers) with very high sensitivity and also facilitates monitoring of receptor-ligand binding in real time. The chip is fabricated in the low fluorescence polymer Zeonor and is compatible with mass production via micro-injection moulding. The performance of the platform is demonstrated by application to a standard bioassay.

AB - A novel, generic lab-on-a-chip platform and associated readout instrumentation is presented. This high-sensitivity system has been developed for the efficient detection of surface-generated fluorescence in biomedical diagnostic applications. The proofof-principle polymer chip contains a 3×3 array of paraboloid elements designed to capture supercritical angle fluorescence (SAF) emitted from biorecognition zones on the top of each paraboloid. Each such element exhibits a fluorescence collection efficiency of 32%, comparable only to sophisticated microscope objectives of high numerical aperture. Furthermore, the chip optical design results in strict confinement of the fluorescence excitation to the surface. Consequently, this inexpensive chip combines the collecting power of modern microscopy optics with strong discrimination between surface and bulk fluorescence. The chip allows for simultaneous detection of 9 different targets (e.g. biomarkers) with very high sensitivity and also facilitates monitoring of receptor-ligand binding in real time. The chip is fabricated in the low fluorescence polymer Zeonor and is compatible with mass production via micro-injection moulding. The performance of the platform is demonstrated by application to a standard bioassay.

KW - highly sensitive

KW - fluorescence detection

KW - biochip

M3 - Abstract

ER -

Ruckstuhl T, Blue R, Spillman S, Kent N, McEvoy H, Laib S et al. Highly sensitive fluorescence detection on a biochip. 2006. Abstract from Pushing the technology envelope III: The next generations of diagnostic testing, Oak Ridge Conference, San Jose, April 2006., San Jose, United States.