Highly-sensitive electrochemical detection of proteins using aptamer-coated gold nanoparticles and surface enzyme reactions

E.J. Nam, E.J. Kim, Alastair Wark, S. Rho, H. Kim, H.J. Lee

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Gold nanoparticle (NP) enhanced surface sandwich assays for the detection of proteins is developed in conjunction with a surface enzyme reaction. As a model protein, immunoglobulin E (IgE) possessing two different epitopes for anti-IgE and IgE specific aptamer is used. A surface sandwich was first formed via the adsorption of IgE onto IgE aptamer coated Au NP-modified gold electrodes followed by the specific interaction of alkaline phosphatase (ALP) conjugated anti-IgE onto the surface IgE complex. The selective electrochemical signal was then achieved by measuring released electrons from the reaction of the substrate, 4-aminophenylphosphate (APP) with the surface IgE-aptamer-NPs/IgE/anti-IgE-ALP complex. The signal enhancement effect of NPs in ALP amplified assays was also studied using the IgE aptamer/IgE/antiIgE-ALP complex. The use of aptamer coated NPs with the enzymatically amplified sandwich assay resulted in an excellent enhancement for IgE detection and a significant reduction of non-specific adsorption events.
LanguageEnglish
Pages2011-2016
Number of pages6
JournalAnalyst
Volume137
DOIs
Publication statusPublished - 2012

Fingerprint

Gold
Nanoparticles
Immunoglobulin E
Phosphatases
Enzymes
gold
phosphatase
enzyme
Proteins
protein
Assays
assay
adsorption
Epitopes
Adsorption
Alkaline Phosphatase
B-Cell Antigen Receptors
electrode
nanoparticle
detection

Keywords

  • electrochemical detection
  • proteins
  • aptamer-coated gold
  • nanoparticles
  • enzyme reactions

Cite this

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title = "Highly-sensitive electrochemical detection of proteins using aptamer-coated gold nanoparticles and surface enzyme reactions",
abstract = "Gold nanoparticle (NP) enhanced surface sandwich assays for the detection of proteins is developed in conjunction with a surface enzyme reaction. As a model protein, immunoglobulin E (IgE) possessing two different epitopes for anti-IgE and IgE specific aptamer is used. A surface sandwich was first formed via the adsorption of IgE onto IgE aptamer coated Au NP-modified gold electrodes followed by the specific interaction of alkaline phosphatase (ALP) conjugated anti-IgE onto the surface IgE complex. The selective electrochemical signal was then achieved by measuring released electrons from the reaction of the substrate, 4-aminophenylphosphate (APP) with the surface IgE-aptamer-NPs/IgE/anti-IgE-ALP complex. The signal enhancement effect of NPs in ALP amplified assays was also studied using the IgE aptamer/IgE/antiIgE-ALP complex. The use of aptamer coated NPs with the enzymatically amplified sandwich assay resulted in an excellent enhancement for IgE detection and a significant reduction of non-specific adsorption events.",
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Highly-sensitive electrochemical detection of proteins using aptamer-coated gold nanoparticles and surface enzyme reactions. / Nam, E.J.; Kim, E.J.; Wark, Alastair; Rho, S.; Kim, H.; Lee, H.J.

In: Analyst, Vol. 137, 2012, p. 2011-2016.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Highly-sensitive electrochemical detection of proteins using aptamer-coated gold nanoparticles and surface enzyme reactions

AU - Nam, E.J.

AU - Kim, E.J.

AU - Wark, Alastair

AU - Rho, S.

AU - Kim, H.

AU - Lee, H.J.

PY - 2012

Y1 - 2012

N2 - Gold nanoparticle (NP) enhanced surface sandwich assays for the detection of proteins is developed in conjunction with a surface enzyme reaction. As a model protein, immunoglobulin E (IgE) possessing two different epitopes for anti-IgE and IgE specific aptamer is used. A surface sandwich was first formed via the adsorption of IgE onto IgE aptamer coated Au NP-modified gold electrodes followed by the specific interaction of alkaline phosphatase (ALP) conjugated anti-IgE onto the surface IgE complex. The selective electrochemical signal was then achieved by measuring released electrons from the reaction of the substrate, 4-aminophenylphosphate (APP) with the surface IgE-aptamer-NPs/IgE/anti-IgE-ALP complex. The signal enhancement effect of NPs in ALP amplified assays was also studied using the IgE aptamer/IgE/antiIgE-ALP complex. The use of aptamer coated NPs with the enzymatically amplified sandwich assay resulted in an excellent enhancement for IgE detection and a significant reduction of non-specific adsorption events.

AB - Gold nanoparticle (NP) enhanced surface sandwich assays for the detection of proteins is developed in conjunction with a surface enzyme reaction. As a model protein, immunoglobulin E (IgE) possessing two different epitopes for anti-IgE and IgE specific aptamer is used. A surface sandwich was first formed via the adsorption of IgE onto IgE aptamer coated Au NP-modified gold electrodes followed by the specific interaction of alkaline phosphatase (ALP) conjugated anti-IgE onto the surface IgE complex. The selective electrochemical signal was then achieved by measuring released electrons from the reaction of the substrate, 4-aminophenylphosphate (APP) with the surface IgE-aptamer-NPs/IgE/anti-IgE-ALP complex. The signal enhancement effect of NPs in ALP amplified assays was also studied using the IgE aptamer/IgE/antiIgE-ALP complex. The use of aptamer coated NPs with the enzymatically amplified sandwich assay resulted in an excellent enhancement for IgE detection and a significant reduction of non-specific adsorption events.

KW - electrochemical detection

KW - proteins

KW - aptamer-coated gold

KW - nanoparticles

KW - enzyme reactions

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U2 - 10.1039/c2an15994e

DO - 10.1039/c2an15994e

M3 - Article

VL - 137

SP - 2011

EP - 2016

JO - Analyst

T2 - Analyst

JF - Analyst

SN - 0003-2654

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