Highly sensitive detection of NADH using electrochemiluminescent nanocomposites

Anitha Devadoss, Lynn Dennany, Calum Dickinson, Tia E Keyes, Robert J. Forster

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Electrodes, modified with [Ru(bpy)2PVP10](ClO4)2 metallopolymer:gold nanoparticle composites, are used for the high sensitivity detection of nicotinamide adenine dinucleotide, NADH, using electrochemiluminescence, ECL; bpy is 2,2-bipyridyl and PVP is poly (4-vinylpyridine). Incorporation of 12.5 nm gold nanoparticles protected with 4-dimethylaminopyridine, DMAP-AuNPs, causes the overall ECL efficiency to decrease with increasing nanoparticle loading. However, the absolute emission intensity and signal-to-noise ratio are both higher for the nanocomposite than the parent metallopolymer. The ECL intensity depends linearly on [NADH] from 10 fM to 10 M with a limit of detection, LOD, of 5 fM. The wide dynamic range and low limit of detection makes these nanocomposite films attractive for biosensor development.
LanguageEnglish
Pages43-45
Number of pages3
JournalElectrochemistry Communications
Volume19
Early online date10 Mar 2012
DOIs
Publication statusPublished - 10 May 2012

Fingerprint

Emitter coupled logic circuits
NAD
Nanocomposites
Nanoparticles
Gold
2,2'-Dipyridyl
Nanocomposite films
Biosensors
Signal to noise ratio
Electrodes
Composite materials

Keywords

  • nanocomposite
  • electrochemiluminescence
  • modified electrode
  • metal enhanced luminescence;
  • metallopolymer

Cite this

Devadoss, Anitha ; Dennany, Lynn ; Dickinson, Calum ; Keyes, Tia E ; Forster, Robert J. / Highly sensitive detection of NADH using electrochemiluminescent nanocomposites. In: Electrochemistry Communications. 2012 ; Vol. 19. pp. 43-45.
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Highly sensitive detection of NADH using electrochemiluminescent nanocomposites. / Devadoss, Anitha; Dennany, Lynn; Dickinson, Calum; Keyes, Tia E; Forster, Robert J.

In: Electrochemistry Communications, Vol. 19, 10.05.2012, p. 43-45.

Research output: Contribution to journalArticle

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AB - Electrodes, modified with [Ru(bpy)2PVP10](ClO4)2 metallopolymer:gold nanoparticle composites, are used for the high sensitivity detection of nicotinamide adenine dinucleotide, NADH, using electrochemiluminescence, ECL; bpy is 2,2-bipyridyl and PVP is poly (4-vinylpyridine). Incorporation of 12.5 nm gold nanoparticles protected with 4-dimethylaminopyridine, DMAP-AuNPs, causes the overall ECL efficiency to decrease with increasing nanoparticle loading. However, the absolute emission intensity and signal-to-noise ratio are both higher for the nanocomposite than the parent metallopolymer. The ECL intensity depends linearly on [NADH] from 10 fM to 10 M with a limit of detection, LOD, of 5 fM. The wide dynamic range and low limit of detection makes these nanocomposite films attractive for biosensor development.

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