Highly sensitive detection of NADH using electrochemiluminescent nanocomposites

Anitha Devadoss; Lynn Dennany; Calum Dickinson; Tia E Keyes; Robert J. Forster

doi:10.1016/j.elecom.2012.03.003

Highly sensitive detection of NADH using electrochemiluminescent nanocomposites

Anitha Devadoss, Lynn Dennany, Calum Dickinson, Tia E Keyes, Robert J. Forster

Pure And Applied Chemistry

Research output: Contribution to journal › Article › peer-review

33 Citations (Scopus)

Abstract

Electrodes, modified with [Ru(bpy)2PVP10](ClO4)2 metallopolymer:gold nanoparticle composites, are used for the high sensitivity detection of nicotinamide adenine dinucleotide, NADH, using electrochemiluminescence, ECL; bpy is 2,2-bipyridyl and PVP is poly (4-vinylpyridine). Incorporation of 12.5 nm gold nanoparticles protected with 4-dimethylaminopyridine, DMAP-AuNPs, causes the overall ECL efficiency to decrease with increasing nanoparticle loading. However, the absolute emission intensity and signal-to-noise ratio are both higher for the nanocomposite than the parent metallopolymer. The ECL intensity depends linearly on [NADH] from 10 fM to 10 M with a limit of detection, LOD, of 5 fM. The wide dynamic range and low limit of detection makes these nanocomposite films attractive for biosensor development.

Original language	English
Pages (from-to)	43-45
Number of pages	3
Journal	Electrochemistry Communications
Volume	19
Early online date	10 Mar 2012
DOIs	https://doi.org/10.1016/j.elecom.2012.03.003
Publication status	Published - 10 May 2012

Keywords

nanocomposite
electrochemiluminescence
modified electrode
metal enhanced luminescence;
metallopolymer

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title = "Highly sensitive detection of NADH using electrochemiluminescent nanocomposites",

abstract = "Electrodes, modified with [Ru(bpy)2PVP10](ClO4)2 metallopolymer:gold nanoparticle composites, are used for the high sensitivity detection of nicotinamide adenine dinucleotide, NADH, using electrochemiluminescence, ECL; bpy is 2,2-bipyridyl and PVP is poly (4-vinylpyridine). Incorporation of 12.5 nm gold nanoparticles protected with 4-dimethylaminopyridine, DMAP-AuNPs, causes the overall ECL efficiency to decrease with increasing nanoparticle loading. However, the absolute emission intensity and signal-to-noise ratio are both higher for the nanocomposite than the parent metallopolymer. The ECL intensity depends linearly on [NADH] from 10 fM to 10 M with a limit of detection, LOD, of 5 fM. The wide dynamic range and low limit of detection makes these nanocomposite films attractive for biosensor development.",

keywords = "nanocomposite , electrochemiluminescence, modified electrode, metal enhanced luminescence; , metallopolymer",

author = "Anitha Devadoss and Lynn Dennany and Calum Dickinson and Keyes, {Tia E} and Forster, {Robert J.}",

year = "2012",

month = may,

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doi = "10.1016/j.elecom.2012.03.003",

language = "English",

volume = "19",

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journal = "Electrochemistry Communications",

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T1 - Highly sensitive detection of NADH using electrochemiluminescent nanocomposites

AU - Devadoss, Anitha

AU - Dennany, Lynn

AU - Dickinson, Calum

AU - Keyes, Tia E

AU - Forster, Robert J.

PY - 2012/5/10

Y1 - 2012/5/10

N2 - Electrodes, modified with [Ru(bpy)2PVP10](ClO4)2 metallopolymer:gold nanoparticle composites, are used for the high sensitivity detection of nicotinamide adenine dinucleotide, NADH, using electrochemiluminescence, ECL; bpy is 2,2-bipyridyl and PVP is poly (4-vinylpyridine). Incorporation of 12.5 nm gold nanoparticles protected with 4-dimethylaminopyridine, DMAP-AuNPs, causes the overall ECL efficiency to decrease with increasing nanoparticle loading. However, the absolute emission intensity and signal-to-noise ratio are both higher for the nanocomposite than the parent metallopolymer. The ECL intensity depends linearly on [NADH] from 10 fM to 10 M with a limit of detection, LOD, of 5 fM. The wide dynamic range and low limit of detection makes these nanocomposite films attractive for biosensor development.

AB - Electrodes, modified with [Ru(bpy)2PVP10](ClO4)2 metallopolymer:gold nanoparticle composites, are used for the high sensitivity detection of nicotinamide adenine dinucleotide, NADH, using electrochemiluminescence, ECL; bpy is 2,2-bipyridyl and PVP is poly (4-vinylpyridine). Incorporation of 12.5 nm gold nanoparticles protected with 4-dimethylaminopyridine, DMAP-AuNPs, causes the overall ECL efficiency to decrease with increasing nanoparticle loading. However, the absolute emission intensity and signal-to-noise ratio are both higher for the nanocomposite than the parent metallopolymer. The ECL intensity depends linearly on [NADH] from 10 fM to 10 M with a limit of detection, LOD, of 5 fM. The wide dynamic range and low limit of detection makes these nanocomposite films attractive for biosensor development.

KW - nanocomposite

KW - electrochemiluminescence

KW - modified electrode

KW - metal enhanced luminescence;

KW - metallopolymer

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DO - 10.1016/j.elecom.2012.03.003

M3 - Article

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VL - 19

SP - 43

EP - 45

JO - Electrochemistry Communications

JF - Electrochemistry Communications

ER -

Highly sensitive detection of NADH using electrochemiluminescent nanocomposites

Abstract

Keywords

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