Highly sensitive detection of NADH using electrochemiluminescent nanocomposites

Anitha Devadoss, Lynn Dennany, Calum Dickinson, Tia E Keyes, Robert J. Forster

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Electrodes, modified with [Ru(bpy)2PVP10](ClO4)2 metallopolymer:gold nanoparticle composites, are used for the high sensitivity detection of nicotinamide adenine dinucleotide, NADH, using electrochemiluminescence, ECL; bpy is 2,2-bipyridyl and PVP is poly (4-vinylpyridine). Incorporation of 12.5 nm gold nanoparticles protected with 4-dimethylaminopyridine, DMAP-AuNPs, causes the overall ECL efficiency to decrease with increasing nanoparticle loading. However, the absolute emission intensity and signal-to-noise ratio are both higher for the nanocomposite than the parent metallopolymer. The ECL intensity depends linearly on [NADH] from 10 fM to 10 M with a limit of detection, LOD, of 5 fM. The wide dynamic range and low limit of detection makes these nanocomposite films attractive for biosensor development.
Original languageEnglish
Pages (from-to)43-45
Number of pages3
JournalElectrochemistry Communications
Volume19
Early online date10 Mar 2012
DOIs
Publication statusPublished - 10 May 2012

Keywords

  • nanocomposite
  • electrochemiluminescence
  • modified electrode
  • metal enhanced luminescence;
  • metallopolymer

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