Highly active protein surfaces enabled by plant-based polyphenol coatings

Ana M. L. Sousa, Tai-De Li, Sabu Varghese, Peter J. Halling, King Hang Aaron Lau

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Proteins represent complex biomolecules capable of wide-ranging but also highly specific functionalities. Their immobilization on material supports can enable broad applications from sensing and industrial biocatalysis to biomedical interfaces and materials. We demonstrate the advantages of using aqueous-processed cross-linked polyphenol coatings for immobilizing proteins, including IgG, avidin, and various single and multidomain enzymes on diverse materials, to enable active biofunctional structures (e.g., ca. 2.2, 1.7, 1.1, and 4.8 mg·m–2 active phosphatase on nanoporous cellulose and alumina, steel mesh, and polyester fabric, respectively). Enzyme assays, X-ray photoelectron spectroscopy, silver staining, supplemented with contact angle, solid-state 13C NMR, HPLC, and ESI-MS measurements were used to characterize the polyphenols, coatings, and protein layers. We show that the functionalization process may be advantageously optimized directly for protein activity rather than the traditional focus on the thickness of the coating layer. Higher activities (by more than an order of magnitude in some cases) and wider process pH and material compatibility are demonstrated with polyphenol coatings than other approaches such as polydopamine. Coatings formed from different plant polyphenol extracts, even at lowered purity (and cost), were also found to be highly functional. Chemically, our results indicate that polyphenol coatings differ from polydopamine mainly because of the elimination of amine groups, and that polyphenol layers with intermediate levels of reactivity may better lead to high immobilized protein activity. Overall, an improved understanding of simple-to-use polyphenol coatings has been obtained, which enabled a significant development in active protein surfaces that may be applied across diverse materials and nanostructured supports.
LanguageEnglish
Pages39353-39362
Number of pages10
JournalACS Applied Materials and Interfaces
Volume10
Issue number45
Early online date9 Oct 2018
DOIs
Publication statusPublished - 14 Nov 2018

Fingerprint

Polyphenols
Membrane Proteins
Proteins
Coatings
Enzymes
Biocatalysis
Immobilized Proteins
Photoelectron Spectroscopy
Silver Staining
Polyesters
Avidin
Nanostructures
Aluminum Oxide
Steel
Phosphatases
Plant Extracts
Enzyme Assays
Biomolecules
Silver
Phosphoric Monoester Hydrolases

Keywords

  • immobilisation
  • enzymes
  • proteins
  • polyphenols
  • tannic acid
  • XPS
  • alumina
  • cellulose
  • nanoporous materials
  • surface functionalisation

Cite this

@article{cf51a2663a0c4253b76214e9941859c3,
title = "Highly active protein surfaces enabled by plant-based polyphenol coatings",
abstract = "Proteins represent complex biomolecules capable of wide-ranging but also highly specific functionalities. Their immobilization on material supports can enable broad applications from sensing and industrial biocatalysis to biomedical interfaces and materials. We demonstrate the advantages of using aqueous-processed cross-linked polyphenol coatings for immobilizing proteins, including IgG, avidin, and various single and multidomain enzymes on diverse materials, to enable active biofunctional structures (e.g., ca. 2.2, 1.7, 1.1, and 4.8 mg·m–2 active phosphatase on nanoporous cellulose and alumina, steel mesh, and polyester fabric, respectively). Enzyme assays, X-ray photoelectron spectroscopy, silver staining, supplemented with contact angle, solid-state 13C NMR, HPLC, and ESI-MS measurements were used to characterize the polyphenols, coatings, and protein layers. We show that the functionalization process may be advantageously optimized directly for protein activity rather than the traditional focus on the thickness of the coating layer. Higher activities (by more than an order of magnitude in some cases) and wider process pH and material compatibility are demonstrated with polyphenol coatings than other approaches such as polydopamine. Coatings formed from different plant polyphenol extracts, even at lowered purity (and cost), were also found to be highly functional. Chemically, our results indicate that polyphenol coatings differ from polydopamine mainly because of the elimination of amine groups, and that polyphenol layers with intermediate levels of reactivity may better lead to high immobilized protein activity. Overall, an improved understanding of simple-to-use polyphenol coatings has been obtained, which enabled a significant development in active protein surfaces that may be applied across diverse materials and nanostructured supports.",
keywords = "immobilisation, enzymes, proteins, polyphenols, tannic acid, XPS, alumina, cellulose, nanoporous materials, surface functionalisation",
author = "Sousa, {Ana M. L.} and Tai-De Li and Sabu Varghese and Halling, {Peter J.} and Lau, {King Hang Aaron}",
year = "2018",
month = "11",
day = "14",
doi = "10.1021/acsami.8b13793",
language = "English",
volume = "10",
pages = "39353--39362",
journal = "ACS Applied Materials and Interfaces",
issn = "1944-8244",
publisher = "American Chemical Society",
number = "45",

}

Highly active protein surfaces enabled by plant-based polyphenol coatings. / Sousa, Ana M. L.; Li, Tai-De; Varghese, Sabu; Halling, Peter J.; Lau, King Hang Aaron.

In: ACS Applied Materials and Interfaces, Vol. 10, No. 45, 14.11.2018, p. 39353-39362.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Highly active protein surfaces enabled by plant-based polyphenol coatings

AU - Sousa, Ana M. L.

AU - Li, Tai-De

AU - Varghese, Sabu

AU - Halling, Peter J.

AU - Lau, King Hang Aaron

PY - 2018/11/14

Y1 - 2018/11/14

N2 - Proteins represent complex biomolecules capable of wide-ranging but also highly specific functionalities. Their immobilization on material supports can enable broad applications from sensing and industrial biocatalysis to biomedical interfaces and materials. We demonstrate the advantages of using aqueous-processed cross-linked polyphenol coatings for immobilizing proteins, including IgG, avidin, and various single and multidomain enzymes on diverse materials, to enable active biofunctional structures (e.g., ca. 2.2, 1.7, 1.1, and 4.8 mg·m–2 active phosphatase on nanoporous cellulose and alumina, steel mesh, and polyester fabric, respectively). Enzyme assays, X-ray photoelectron spectroscopy, silver staining, supplemented with contact angle, solid-state 13C NMR, HPLC, and ESI-MS measurements were used to characterize the polyphenols, coatings, and protein layers. We show that the functionalization process may be advantageously optimized directly for protein activity rather than the traditional focus on the thickness of the coating layer. Higher activities (by more than an order of magnitude in some cases) and wider process pH and material compatibility are demonstrated with polyphenol coatings than other approaches such as polydopamine. Coatings formed from different plant polyphenol extracts, even at lowered purity (and cost), were also found to be highly functional. Chemically, our results indicate that polyphenol coatings differ from polydopamine mainly because of the elimination of amine groups, and that polyphenol layers with intermediate levels of reactivity may better lead to high immobilized protein activity. Overall, an improved understanding of simple-to-use polyphenol coatings has been obtained, which enabled a significant development in active protein surfaces that may be applied across diverse materials and nanostructured supports.

AB - Proteins represent complex biomolecules capable of wide-ranging but also highly specific functionalities. Their immobilization on material supports can enable broad applications from sensing and industrial biocatalysis to biomedical interfaces and materials. We demonstrate the advantages of using aqueous-processed cross-linked polyphenol coatings for immobilizing proteins, including IgG, avidin, and various single and multidomain enzymes on diverse materials, to enable active biofunctional structures (e.g., ca. 2.2, 1.7, 1.1, and 4.8 mg·m–2 active phosphatase on nanoporous cellulose and alumina, steel mesh, and polyester fabric, respectively). Enzyme assays, X-ray photoelectron spectroscopy, silver staining, supplemented with contact angle, solid-state 13C NMR, HPLC, and ESI-MS measurements were used to characterize the polyphenols, coatings, and protein layers. We show that the functionalization process may be advantageously optimized directly for protein activity rather than the traditional focus on the thickness of the coating layer. Higher activities (by more than an order of magnitude in some cases) and wider process pH and material compatibility are demonstrated with polyphenol coatings than other approaches such as polydopamine. Coatings formed from different plant polyphenol extracts, even at lowered purity (and cost), were also found to be highly functional. Chemically, our results indicate that polyphenol coatings differ from polydopamine mainly because of the elimination of amine groups, and that polyphenol layers with intermediate levels of reactivity may better lead to high immobilized protein activity. Overall, an improved understanding of simple-to-use polyphenol coatings has been obtained, which enabled a significant development in active protein surfaces that may be applied across diverse materials and nanostructured supports.

KW - immobilisation

KW - enzymes

KW - proteins

KW - polyphenols

KW - tannic acid

KW - XPS

KW - alumina

KW - cellulose

KW - nanoporous materials

KW - surface functionalisation

UR - https://pubs.acs.org/journal/aamick

U2 - 10.1021/acsami.8b13793

DO - 10.1021/acsami.8b13793

M3 - Article

VL - 10

SP - 39353

EP - 39362

JO - ACS Applied Materials and Interfaces

T2 - ACS Applied Materials and Interfaces

JF - ACS Applied Materials and Interfaces

SN - 1944-8244

IS - 45

ER -