High throughput optofluidic Surface-Enhanced Raman Spectroscopy (SERS) interrogation: proof of concept via lectin detection of cancerous cells

Marjorie Willner, Jonathan Simpson, Kay McMillan, Michele Zagnoni, Duncan Graham, Peter Vikesland

Research output: Contribution to conferenceAbstract

Abstract

High throughput surface enhanced Raman spectroscopy (SERS) is a much needed advance to better realize the full potential of the detailed fingerprint information available with Raman spectroscopy. At present SERS, as an analytical technique, is often plagued by a lack of quantification and reproducibility due to the length of time required for individual sample analysis. In this effort we discuss the development of an optofluidic device that combines the high throughput capability of microfluidics with SERS detection. A two-phase microfluidic based platform with droplet storage allows for the rapid screening of over one hundred individual prostate cells. Specifically, the N-acetyl neuraminic (sialic) acid residue overexpressed on cancerous prostate cells (PC3), compared to non-cancerous cells (PNT2), is targeted with carbohydrate (lectin) functionalized nanoparticles. In-house algorithms were developed to automatically process SERS maps and rapidly distinguish cancerous PC3 cells from both non-cancerous PNT2A cells and empty droplets. Furthermore, the computer vision methods employed are scalable. Herein, we demonstrate the novel use of labeled nanoparticles for SERS detection in a high throughput segmented flow optofluidic device.
LanguageEnglish
Publication statusPublished - 27 Sep 2015
EventSciX 2015 - USA, Providence, RI, United Kingdom
Duration: 27 Sep 20152 Oct 2015

Conference

ConferenceSciX 2015
CountryUnited Kingdom
CityProvidence, RI
Period27/09/152/10/15

Fingerprint

interrogation
Lectins
Raman spectroscopy
Throughput
cells
Microfluidics
Nanoparticles
nanoparticles
carbohydrates
computer vision
N-Acetylneuraminic Acid
Computer vision
Screening
screening
platforms
Carbohydrates
acids

Keywords

  • Surface-Enhanced Raman Spectroscopy
  • SERS
  • lectin detection
  • optofluidic devices

Cite this

Willner, M., Simpson, J., McMillan, K., Zagnoni, M., Graham, D., & Vikesland, P. (2015). High throughput optofluidic Surface-Enhanced Raman Spectroscopy (SERS) interrogation: proof of concept via lectin detection of cancerous cells. Abstract from SciX 2015, Providence, RI, United Kingdom.
Willner, Marjorie ; Simpson, Jonathan ; McMillan, Kay ; Zagnoni, Michele ; Graham, Duncan ; Vikesland, Peter. / High throughput optofluidic Surface-Enhanced Raman Spectroscopy (SERS) interrogation : proof of concept via lectin detection of cancerous cells. Abstract from SciX 2015, Providence, RI, United Kingdom.
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Willner, M, Simpson, J, McMillan, K, Zagnoni, M, Graham, D & Vikesland, P 2015, 'High throughput optofluidic Surface-Enhanced Raman Spectroscopy (SERS) interrogation: proof of concept via lectin detection of cancerous cells' SciX 2015, Providence, RI, United Kingdom, 27/09/15 - 2/10/15, .

High throughput optofluidic Surface-Enhanced Raman Spectroscopy (SERS) interrogation : proof of concept via lectin detection of cancerous cells. / Willner, Marjorie; Simpson, Jonathan; McMillan, Kay; Zagnoni, Michele; Graham, Duncan; Vikesland, Peter.

2015. Abstract from SciX 2015, Providence, RI, United Kingdom.

Research output: Contribution to conferenceAbstract

TY - CONF

T1 - High throughput optofluidic Surface-Enhanced Raman Spectroscopy (SERS) interrogation

T2 - proof of concept via lectin detection of cancerous cells

AU - Willner, Marjorie

AU - Simpson, Jonathan

AU - McMillan, Kay

AU - Zagnoni, Michele

AU - Graham, Duncan

AU - Vikesland, Peter

PY - 2015/9/27

Y1 - 2015/9/27

N2 - High throughput surface enhanced Raman spectroscopy (SERS) is a much needed advance to better realize the full potential of the detailed fingerprint information available with Raman spectroscopy. At present SERS, as an analytical technique, is often plagued by a lack of quantification and reproducibility due to the length of time required for individual sample analysis. In this effort we discuss the development of an optofluidic device that combines the high throughput capability of microfluidics with SERS detection. A two-phase microfluidic based platform with droplet storage allows for the rapid screening of over one hundred individual prostate cells. Specifically, the N-acetyl neuraminic (sialic) acid residue overexpressed on cancerous prostate cells (PC3), compared to non-cancerous cells (PNT2), is targeted with carbohydrate (lectin) functionalized nanoparticles. In-house algorithms were developed to automatically process SERS maps and rapidly distinguish cancerous PC3 cells from both non-cancerous PNT2A cells and empty droplets. Furthermore, the computer vision methods employed are scalable. Herein, we demonstrate the novel use of labeled nanoparticles for SERS detection in a high throughput segmented flow optofluidic device.

AB - High throughput surface enhanced Raman spectroscopy (SERS) is a much needed advance to better realize the full potential of the detailed fingerprint information available with Raman spectroscopy. At present SERS, as an analytical technique, is often plagued by a lack of quantification and reproducibility due to the length of time required for individual sample analysis. In this effort we discuss the development of an optofluidic device that combines the high throughput capability of microfluidics with SERS detection. A two-phase microfluidic based platform with droplet storage allows for the rapid screening of over one hundred individual prostate cells. Specifically, the N-acetyl neuraminic (sialic) acid residue overexpressed on cancerous prostate cells (PC3), compared to non-cancerous cells (PNT2), is targeted with carbohydrate (lectin) functionalized nanoparticles. In-house algorithms were developed to automatically process SERS maps and rapidly distinguish cancerous PC3 cells from both non-cancerous PNT2A cells and empty droplets. Furthermore, the computer vision methods employed are scalable. Herein, we demonstrate the novel use of labeled nanoparticles for SERS detection in a high throughput segmented flow optofluidic device.

KW - Surface-Enhanced Raman Spectroscopy

KW - SERS

KW - lectin detection

KW - optofluidic devices

M3 - Abstract

ER -

Willner M, Simpson J, McMillan K, Zagnoni M, Graham D, Vikesland P. High throughput optofluidic Surface-Enhanced Raman Spectroscopy (SERS) interrogation: proof of concept via lectin detection of cancerous cells. 2015. Abstract from SciX 2015, Providence, RI, United Kingdom.