High-resolution orthogonal techniques for separating and detecting impurities in antisense oligonucleotide therapeutics

Callum G. Davidson; Panida Punnabhum; Gillian Farrell; Yvonne Perrie; Zahra Rattray

High-resolution orthogonal techniques for separating and detecting impurities in antisense oligonucleotide therapeutics

Callum G. Davidson, Panida Punnabhum, Gillian Farrell, Yvonne Perrie, Zahra Rattray^*

^*Corresponding author for this work

Strathclyde Institute Of Pharmacy And Biomedical Sciences

Research output: Contribution to conference › Presentation/Speech

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Abstract

Aim:
The aim of this study is to develop an orthogonal analytical pipeline for the analysis of
manufacture associated impurities and RNA API critical quality attributes using
Nusinersen.

Objectives:
• Develop and optimise hydrophilic interaction liquid chromatography.
• Develop orthogonal analytical method pipeline to evaluate antisense oligonucleotide
beyond the scope of routine techniques.
• Compare results between orthogonal high resolution analytical techniques.

Original language	English
Pages	15
Publication status	Published - 12 Nov 2024
Event	JPAG Pharmaceutical Analysis Research Awards and Careers Fair 2024 - London, United Kingdom Duration: 12 Nov 2024 → 12 Nov 2024 https://www.jpag.org/?p=meetings&r=174

Conference

Conference	JPAG Pharmaceutical Analysis Research Awards and Careers Fair 2024
Country/Territory	United Kingdom
City	London
Period	12/11/24 → 12/11/24
Internet address	https://www.jpag.org/?p=meetings&r=174

Keywords

RNA
EAF4
chromatographic analysis
impurity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

Davidson-etal-JPAG-2024-separating-and-detecting-impurities-in-antisense-oligonucleotide-therapeuticsFinal published version, 1.36 MBLicence: Strath_1

Cite this

@conference{5737687b227647fa8c2954bcde89db1e,

title = "High-resolution orthogonal techniques for separating and detecting impurities in antisense oligonucleotide therapeutics",

abstract = "Aim:The aim of this study is to develop an orthogonal analytical pipeline for the analysis ofmanufacture associated impurities and RNA API critical quality attributes usingNusinersen.Objectives:• Develop and optimise hydrophilic interaction liquid chromatography.• Develop orthogonal analytical method pipeline to evaluate antisense oligonucleotidebeyond the scope of routine techniques.• Compare results between orthogonal high resolution analytical techniques.",

keywords = "RNA, EAF4, chromatographic analysis, impurity",

author = "Davidson, {Callum G.} and Panida Punnabhum and Gillian Farrell and Yvonne Perrie and Zahra Rattray",

year = "2024",

month = nov,

day = "12",

language = "English",

pages = "15",

note = "JPAG Pharmaceutical Analysis Research Awards and Careers Fair 2024 ; Conference date: 12-11-2024 Through 12-11-2024",

url = "https://www.jpag.org/?p=meetings&r=174",

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TY - CONF

T1 - High-resolution orthogonal techniques for separating and detecting impurities in antisense oligonucleotide therapeutics

AU - Davidson, Callum G.

AU - Punnabhum, Panida

AU - Farrell, Gillian

AU - Perrie, Yvonne

AU - Rattray, Zahra

PY - 2024/11/12

Y1 - 2024/11/12

N2 - Aim:The aim of this study is to develop an orthogonal analytical pipeline for the analysis ofmanufacture associated impurities and RNA API critical quality attributes usingNusinersen.Objectives:• Develop and optimise hydrophilic interaction liquid chromatography.• Develop orthogonal analytical method pipeline to evaluate antisense oligonucleotidebeyond the scope of routine techniques.• Compare results between orthogonal high resolution analytical techniques.

AB - Aim:The aim of this study is to develop an orthogonal analytical pipeline for the analysis ofmanufacture associated impurities and RNA API critical quality attributes usingNusinersen.Objectives:• Develop and optimise hydrophilic interaction liquid chromatography.• Develop orthogonal analytical method pipeline to evaluate antisense oligonucleotidebeyond the scope of routine techniques.• Compare results between orthogonal high resolution analytical techniques.

KW - RNA

KW - EAF4

KW - chromatographic analysis

KW - impurity

UR - https://www.jpag.org/?p=meetings&r=174

M3 - Presentation/Speech

SP - 15

T2 - JPAG Pharmaceutical Analysis Research Awards and Careers Fair 2024

Y2 - 12 November 2024 through 12 November 2024

ER -

High-resolution orthogonal techniques for separating and detecting impurities in antisense oligonucleotide therapeutics

Abstract

Conference

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

DTP 2224 University of Strathclyde | Davidson, Callum

FFF

Cite this

High-resolution orthogonal techniques for separating and detecting impurities in antisense oligonucleotide therapeutics

Abstract

Conference

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

DTP 2224 University of Strathclyde | Davidson, Callum

Equipment

FFF

Cite this