Hexamer oligonucleotide topology and assembly under solution phase NMR and theoretical modeling scrutiny

Maxim P. Evstigneev, John A. Parkinson, Anastasia O. Lantushenko, Viktor V. Kostjukov, Valery I. Pahomov

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Abstract

The entire family of non-complementary hexamer oligodeoxyribonucleotides d(GCXYGC) (X and Y = A, G, C, or T) were assessed for topological indicators and equilibrium thermodynamics using a priori molecular modeling and solution phase NMR spectroscopy. Feasible modeled hairpin structures formed a basis from which solution structure and equilibria for each oligonucleotide were considered. 1H and 31P variable temperature (VT) and concentration dependent NMR data, NMR signal assignments and diffusion parameters led to d(GCGAGC) and d(GCGGGC) being understood as exceptions within the family in terms of self-association and topological character. A mean diffusion coefficient D298K = (2.0 ± 0.07) × 10-10 m2s-1 was evaluated across all hexamers except for d(GCGAGC) (D298K = 1.7 × 10-10 m2s-1) and d(GCGGGC) (D298K = 1.2 × 10-10 m2s-1). Melting under VT analysis (Tm = 323 K) combined with supporting NMR evidence confirmed d(GCGAGC) as the shortest tandem sheared GA mismatched duplex. Diffusion measurements were used to conclude that d(GCGGGC) preferentially exists as the shortest stable quadruplex structure. Thermodynamic analysis of all data led to the assertion that, with the exception of XY = GA and GG, the remaining non-complementary oligonucleotides adopt equilibria between monomer and duplex, contributed to largely by monomer random-coil forms. Contrastingly d(GCGAGC) showed preference for tandem sheared GA mismatch duplex formation with an association constant K = 3.9 × 105 M-1. No direct evidence was acquired for hairpin formation in any instance although its potential existence is considered possible for d(GCGAGC) on the basis of molecular modeling studies.
Original languageEnglish
Pages (from-to)1023-1038
Number of pages16
JournalBiopolymers
Volume93
Issue number12
Early online date8 Jul 2010
DOIs
Publication statusPublished - Dec 2010

Keywords

  • hexamer DNA topology
  • diffusion NMR
  • thermodynamics
  • molecular modeling
  • tandem mismatch

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