Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches

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Abstract

A newly developed hardware-friendly non-iterative fluorescence lifetime imaging (FLIM) analysis method was verified in an FPGA chip. Its performances were also demonstrated on two-photon FLIM images of gold nanorods (GNRs)-Cy5 labelled Hela cells. The results obtained by the proposed method can be presented in a polor plot to be compared to the widely used phasor (Phasor) approach. Combining our method with Phasor will be very useful in FLIM analysis.

Original languageEnglish
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Place of PublicationBellingham, WA
Volume9536
DOIs
Publication statusPublished - 2015
EventAdvanced Microscopy Techniques IV; and Neurophotonics II - Munich, Germany
Duration: 24 Jun 201525 Jun 2015

Conference

ConferenceAdvanced Microscopy Techniques IV; and Neurophotonics II
CountryGermany
CityMunich
Period24/06/1525/06/15

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Keywords

  • center-of-mass method
  • field programmable gate array
  • fluorescence lifetime imaging microscopy (FLIM)
  • gold nanorods
  • time-correlated single-photon counting
  • time-resolved fluorescence imaging
  • two-photon FLIM

Cite this

Li, D., & Chen, Y. (2015). Hardware-friendly bi-exponential fluorescence lifetime imaging algorithms and phasor approaches. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 9536). [95360M] Bellingham, WA. https://doi.org/10.1117/12.2183082