Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution

Guoke Wei; Jun Yu; Jinliang Wang; Peng Gu; David J.S. Birch; Yu Chen

doi:10.1117/1.JBO.21.9.097001

Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution

Guoke Wei, Jun Yu, Jinliang Wang, Peng Gu, David J.S. Birch, Yu Chen

Research output: Contribution to journal › Article › peer-review

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Abstract

We report a novel fluorescent probe for mRNA detection. It consists of a gold nanorod (GNR) functionalized with fluorophore labeled hairpin oligonucleotides (hpDNA) that are complementary to the mRNA of a target gene. This nanoprobe was found to be sensitive to a complementary oligonucleotide, as indicated by significant changes in both fluorescence intensity and lifetime. The influence of the surface density of hpDNA on the performance of this nanoprobe was investigated, suggesting that high hybridization efficiency could be achieved at a relatively low surface loading density of hpDNA. However, steady-state fluorescence spectroscopy revealed better overall performance, in terms of sensitivity and detection range, for nanoprobes with higher hairpin coverage. Time-resolved fluorescence lifetime spectroscopy revealed significant lifetime changes of the fluorophore upon hybridization of hpDNA with targets, providing further insight on the hybridization kinetics of the probe as well as the quenching efficiency of GNRs.

Original language	English
Article number	097001
Number of pages	31
Journal	Journal of Biomedical Optics
Volume	21
Issue number	9
DOIs	https://doi.org/10.1117/1.JBO.21.9.097001
Publication status	Published - 8 Sept 2016

Keywords

gold nanorods
hairpin DNA
mRNA detections
fluorescence lifetime
fluorescent probe
mRNA detection
fluorophore labeled hairpin oligonucleotides
nanoprobe
hybridization efficiency

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10.1117/1.JBO.21.9.097001

Wei-etal-JBO2016-Hairpin-DNA-functionalized-gold-nanorods-for-mRNA-detectionAccepted author manuscript, 477 KB

Cite this

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title = "Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution",

abstract = "We report a novel fluorescent probe for mRNA detection. It consists of a gold nanorod (GNR) functionalized with fluorophore labeled hairpin oligonucleotides (hpDNA) that are complementary to the mRNA of a target gene. This nanoprobe was found to be sensitive to a complementary oligonucleotide, as indicated by significant changes in both fluorescence intensity and lifetime. The influence of the surface density of hpDNA on the performance of this nanoprobe was investigated, suggesting that high hybridization efficiency could be achieved at a relatively low surface loading density of hpDNA. However, steady-state fluorescence spectroscopy revealed better overall performance, in terms of sensitivity and detection range, for nanoprobes with higher hairpin coverage. Time-resolved fluorescence lifetime spectroscopy revealed significant lifetime changes of the fluorophore upon hybridization of hpDNA with targets, providing further insight on the hybridization kinetics of the probe as well as the quenching efficiency of GNRs.",

keywords = "gold nanorods, hairpin DNA, mRNA detections, fluorescence lifetime, fluorescent probe, mRNA detection, fluorophore labeled hairpin oligonucleotides, nanoprobe, hybridization efficiency",

author = "Guoke Wei and Jun Yu and Jinliang Wang and Peng Gu and Birch, \{David J.S.\} and Yu Chen",

note = "Wei, G., Yu, J., Wang, J., Gu, P., Birch, D. J. S., \& Chen, Y. Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution. Journal of Biomedical Optics, JBO 160218RR (2016). Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. ",

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doi = "10.1117/1.JBO.21.9.097001",

language = "English",

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journal = "Journal of Biomedical Optics",

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AU - Chen, Yu

N1 - Wei, G., Yu, J., Wang, J., Gu, P., Birch, D. J. S., & Chen, Y. Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution. Journal of Biomedical Optics, JBO 160218RR (2016). Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

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Y1 - 2016/9/8

N2 - We report a novel fluorescent probe for mRNA detection. It consists of a gold nanorod (GNR) functionalized with fluorophore labeled hairpin oligonucleotides (hpDNA) that are complementary to the mRNA of a target gene. This nanoprobe was found to be sensitive to a complementary oligonucleotide, as indicated by significant changes in both fluorescence intensity and lifetime. The influence of the surface density of hpDNA on the performance of this nanoprobe was investigated, suggesting that high hybridization efficiency could be achieved at a relatively low surface loading density of hpDNA. However, steady-state fluorescence spectroscopy revealed better overall performance, in terms of sensitivity and detection range, for nanoprobes with higher hairpin coverage. Time-resolved fluorescence lifetime spectroscopy revealed significant lifetime changes of the fluorophore upon hybridization of hpDNA with targets, providing further insight on the hybridization kinetics of the probe as well as the quenching efficiency of GNRs.

AB - We report a novel fluorescent probe for mRNA detection. It consists of a gold nanorod (GNR) functionalized with fluorophore labeled hairpin oligonucleotides (hpDNA) that are complementary to the mRNA of a target gene. This nanoprobe was found to be sensitive to a complementary oligonucleotide, as indicated by significant changes in both fluorescence intensity and lifetime. The influence of the surface density of hpDNA on the performance of this nanoprobe was investigated, suggesting that high hybridization efficiency could be achieved at a relatively low surface loading density of hpDNA. However, steady-state fluorescence spectroscopy revealed better overall performance, in terms of sensitivity and detection range, for nanoprobes with higher hairpin coverage. Time-resolved fluorescence lifetime spectroscopy revealed significant lifetime changes of the fluorophore upon hybridization of hpDNA with targets, providing further insight on the hybridization kinetics of the probe as well as the quenching efficiency of GNRs.

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KW - hybridization efficiency

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Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

A new approach for imaging RNA at the single cell level

NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

Emerging clinical applications of fluorescence using SPADs

Fluorescence guided surgery

Surface plasmon enhanced energy transfer between gold nanorods and fluorophores: application to endosytosis study and RNA detection

Cite this

Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Projects

A new approach for imaging RNA at the single cell level

NMS: EPSRC Science and Innovation Nanometrology for Molecular Science, Medicine and Manufacture

Research output

Emerging clinical applications of fluorescence using SPADs

Fluorescence guided surgery

Surface plasmon enhanced energy transfer between gold nanorods and fluorophores: application to endosytosis study and RNA detection

Cite this