Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution

Guoke Wei, Jun Yu, Jinliang Wang, Peng Gu, David J.S. Birch, Yu Chen

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

We report a novel fluorescent probe for mRNA detection. It consists of a gold nanorod (GNR) functionalized with fluorophore labeled hairpin oligonucleotides (hpDNA) that are complementary to the mRNA of a target gene. This nanoprobe was found to be sensitive to a complementary oligonucleotide, as indicated by significant changes in both fluorescence intensity and lifetime. The influence of the surface density of hpDNA on the performance of this nanoprobe was investigated, suggesting that high hybridization efficiency could be achieved at a relatively low surface loading density of hpDNA. However, steady-state fluorescence spectroscopy revealed better overall performance, in terms of sensitivity and detection range, for nanoprobes with higher hairpin coverage. Time-resolved fluorescence lifetime spectroscopy revealed significant lifetime changes of the fluorophore upon hybridization of hpDNA with targets, providing further insight on the hybridization kinetics of the probe as well as the quenching efficiency of GNRs.
LanguageEnglish
Article number097001
Number of pages31
JournalJournal of Biomedical Optics
Volume 21
Issue number9
DOIs
Publication statusPublished - 8 Sep 2016

Fingerprint

Nanoprobes
Nanorods
Gold
nanorods
DNA
deoxyribonucleic acid
Fluorophores
oligonucleotides
Oligonucleotides
gold
life (durability)
fluorescence
Messenger RNA
Fluorescence
probes
Fluorescence spectroscopy
Fluorescent Dyes
genes
spectroscopy
Quenching

Keywords

  • gold nanorods
  • hairpin DNA
  • mRNA detections
  • fluorescence lifetime
  • fluorescent probe
  • mRNA detection
  • fluorophore labeled hairpin oligonucleotides
  • nanoprobe
  • hybridization efficiency

Cite this

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title = "Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution",
abstract = "We report a novel fluorescent probe for mRNA detection. It consists of a gold nanorod (GNR) functionalized with fluorophore labeled hairpin oligonucleotides (hpDNA) that are complementary to the mRNA of a target gene. This nanoprobe was found to be sensitive to a complementary oligonucleotide, as indicated by significant changes in both fluorescence intensity and lifetime. The influence of the surface density of hpDNA on the performance of this nanoprobe was investigated, suggesting that high hybridization efficiency could be achieved at a relatively low surface loading density of hpDNA. However, steady-state fluorescence spectroscopy revealed better overall performance, in terms of sensitivity and detection range, for nanoprobes with higher hairpin coverage. Time-resolved fluorescence lifetime spectroscopy revealed significant lifetime changes of the fluorophore upon hybridization of hpDNA with targets, providing further insight on the hybridization kinetics of the probe as well as the quenching efficiency of GNRs.",
keywords = "gold nanorods, hairpin DNA, mRNA detections, fluorescence lifetime, fluorescent probe, mRNA detection, fluorophore labeled hairpin oligonucleotides, nanoprobe, hybridization efficiency",
author = "Guoke Wei and Jun Yu and Jinliang Wang and Peng Gu and Birch, {David J.S.} and Yu Chen",
note = "Wei, G., Yu, J., Wang, J., Gu, P., Birch, D. J. S., & Chen, Y. Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution. Journal of Biomedical Optics, JBO 160218RR (2016). Copyright 2016 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.",
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Hairpin DNA functionalized gold nanorods for mRNA detection in homogenous solution. / Wei, Guoke; Yu, Jun; Wang, Jinliang; Gu, Peng; Birch, David J.S.; Chen, Yu.

In: Journal of Biomedical Optics, Vol. 21, No. 9, 097001, 08.09.2016.

Research output: Contribution to journalArticle

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