Growth rate control of flagellar assembly in Escherichia coli strain RP437

Martin Sim, Santosh Koirala, David Picton, Henrik Strahl, Paul A. Hoskisson, Christopher V. Rao, Colin S. Gillespie, Phillip D. Aldridge

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The flagellum is a rotary motor that enables bacteria to swim in liquids and swarm over surfaces. Numerous global regulators control flagellar assembly in response to cellular and environmental factors. Previous studies have also shown that flagellar assembly is affected by the growth-rate of the cell. However, a systematic study has not yet been described under controlled growth conditions. Here, we investigated the effect of growth rate on flagellar assembly in Escherichia coli using steady-state chemostat cultures where we could precisely control the cell growth-rate. Our results demonstrate that flagellar abundance correlates with growth rate, where faster growing cells produce more flagella. They also demonstrate that this growth-rate dependent control occurs through the expression of the flagellar master regulator, FlhD4C2. Collectively, our results demonstrate that motility is intimately coupled to the growth-rate of the cell.

LanguageEnglish
Article number41189
Pages1-11
Number of pages11
JournalScientific Reports
Volume7
DOIs
Publication statusPublished - 24 Jan 2017

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Escherichia coli
Growth
Flagella
Bacteria

Keywords

  • bacteria
  • flagella
  • growth-rate
  • cell

Cite this

Sim, Martin ; Koirala, Santosh ; Picton, David ; Strahl, Henrik ; Hoskisson, Paul A. ; Rao, Christopher V. ; Gillespie, Colin S. ; Aldridge, Phillip D. / Growth rate control of flagellar assembly in Escherichia coli strain RP437. In: Scientific Reports. 2017 ; Vol. 7. pp. 1-11.
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Sim, M, Koirala, S, Picton, D, Strahl, H, Hoskisson, PA, Rao, CV, Gillespie, CS & Aldridge, PD 2017, 'Growth rate control of flagellar assembly in Escherichia coli strain RP437' Scientific Reports, vol. 7, 41189, pp. 1-11. https://doi.org/10.1038/srep41189

Growth rate control of flagellar assembly in Escherichia coli strain RP437. / Sim, Martin; Koirala, Santosh; Picton, David; Strahl, Henrik; Hoskisson, Paul A.; Rao, Christopher V.; Gillespie, Colin S.; Aldridge, Phillip D.

In: Scientific Reports, Vol. 7, 41189, 24.01.2017, p. 1-11.

Research output: Contribution to journalArticle

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T1 - Growth rate control of flagellar assembly in Escherichia coli strain RP437

AU - Sim, Martin

AU - Koirala, Santosh

AU - Picton, David

AU - Strahl, Henrik

AU - Hoskisson, Paul A.

AU - Rao, Christopher V.

AU - Gillespie, Colin S.

AU - Aldridge, Phillip D.

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