Group II metabotropic glutamate receptors depress synaptic transmission onto subicular burst firing neurons

Michael Kintscher, Jörg Breustedt, Stéphanie Miceli, Dietmar Schmitz, Christian Wozny

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The subiculum (SUB) is a pivotal structure positioned between the hippocampus proper and various cortical and subcortical areas. Despite the growing body of anatomical and intrinsic electrophysiological data of subicular neurons, modulation of synaptic transmission in the SUB is not well understood. In the present study we investigated the role of group II metabotropic glutamate receptors (mGluRs), which have been shown to be involved in the regulation of synaptic transmission by suppressing presynaptic cAMP activity. Using field potential and patch-clamp whole cell recordings we demonstrate that glutamatergic transmission at CA1-SUB synapses is depressed by group II mGluRs in a cell-type specific manner. Application of the group II mGluR agonist (2S,1'R,2'R,3'R)-2-(2, 3-dicarboxycyclopropyl)glycine (DCG-IV) led to a significantly higher reduction of excitatory postsynaptic currents in subicular bursting cells than in regular firing cells. We further used low-frequency stimulation protocols and brief high-frequency bursts to test whether synaptically released glutamate is capable of activating presynaptic mGluRs. However, neither frequency facilitation is enhanced in the presence of the group II mGluR antagonist LY341495, nor is a test stimulus given after a high-frequency burst. In summary, we present pharmacological evidence for presynaptic group II mGluRs targeting subicular bursting cells, but both low- and high-frequency stimulation protocols failed to activate presynaptically located mGluRs.

LanguageEnglish
Article numbere45039
Pages1-9
Number of pages9
JournalPLOS One
Volume7
Issue number9
DOIs
Publication statusPublished - 11 Sep 2012

Fingerprint

synaptic transmission
Metabotropic Glutamate Receptors
Synaptic Transmission
Neurons
neurons
Hippocampus
LY 341495
cells
Excitatory Postsynaptic Potentials
Clamping devices
Patch-Clamp Techniques
Synapses
synapse
hippocampus
Glutamic Acid
glycine (amino acid)
glutamates
agonists
Modulation
glutamate receptors

Keywords

  • amino acids
  • amino acids, dicarboxylic
  • animals
  • CA1 region, hippocampal
  • CA3 region, hippocampal
  • cyclopropanes
  • dose-response relationship, drug
  • excitatory amino acid agonists
  • excitatory amino acid antagonists
  • female
  • glutamic acid
  • glycine
  • hippocampus
  • male
  • neurons
  • patch-clamp techniques
  • rats
  • rats, wistar
  • receptors, metabotropic glutamate
  • synaptic transmission
  • xanthenes

Cite this

Kintscher, Michael ; Breustedt, Jörg ; Miceli, Stéphanie ; Schmitz, Dietmar ; Wozny, Christian. / Group II metabotropic glutamate receptors depress synaptic transmission onto subicular burst firing neurons. In: PLOS One. 2012 ; Vol. 7, No. 9. pp. 1-9.
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Group II metabotropic glutamate receptors depress synaptic transmission onto subicular burst firing neurons. / Kintscher, Michael; Breustedt, Jörg; Miceli, Stéphanie; Schmitz, Dietmar; Wozny, Christian.

In: PLOS One, Vol. 7, No. 9, e45039, 11.09.2012, p. 1-9.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Group II metabotropic glutamate receptors depress synaptic transmission onto subicular burst firing neurons

AU - Kintscher, Michael

AU - Breustedt, Jörg

AU - Miceli, Stéphanie

AU - Schmitz, Dietmar

AU - Wozny, Christian

PY - 2012/9/11

Y1 - 2012/9/11

N2 - The subiculum (SUB) is a pivotal structure positioned between the hippocampus proper and various cortical and subcortical areas. Despite the growing body of anatomical and intrinsic electrophysiological data of subicular neurons, modulation of synaptic transmission in the SUB is not well understood. In the present study we investigated the role of group II metabotropic glutamate receptors (mGluRs), which have been shown to be involved in the regulation of synaptic transmission by suppressing presynaptic cAMP activity. Using field potential and patch-clamp whole cell recordings we demonstrate that glutamatergic transmission at CA1-SUB synapses is depressed by group II mGluRs in a cell-type specific manner. Application of the group II mGluR agonist (2S,1'R,2'R,3'R)-2-(2, 3-dicarboxycyclopropyl)glycine (DCG-IV) led to a significantly higher reduction of excitatory postsynaptic currents in subicular bursting cells than in regular firing cells. We further used low-frequency stimulation protocols and brief high-frequency bursts to test whether synaptically released glutamate is capable of activating presynaptic mGluRs. However, neither frequency facilitation is enhanced in the presence of the group II mGluR antagonist LY341495, nor is a test stimulus given after a high-frequency burst. In summary, we present pharmacological evidence for presynaptic group II mGluRs targeting subicular bursting cells, but both low- and high-frequency stimulation protocols failed to activate presynaptically located mGluRs.

AB - The subiculum (SUB) is a pivotal structure positioned between the hippocampus proper and various cortical and subcortical areas. Despite the growing body of anatomical and intrinsic electrophysiological data of subicular neurons, modulation of synaptic transmission in the SUB is not well understood. In the present study we investigated the role of group II metabotropic glutamate receptors (mGluRs), which have been shown to be involved in the regulation of synaptic transmission by suppressing presynaptic cAMP activity. Using field potential and patch-clamp whole cell recordings we demonstrate that glutamatergic transmission at CA1-SUB synapses is depressed by group II mGluRs in a cell-type specific manner. Application of the group II mGluR agonist (2S,1'R,2'R,3'R)-2-(2, 3-dicarboxycyclopropyl)glycine (DCG-IV) led to a significantly higher reduction of excitatory postsynaptic currents in subicular bursting cells than in regular firing cells. We further used low-frequency stimulation protocols and brief high-frequency bursts to test whether synaptically released glutamate is capable of activating presynaptic mGluRs. However, neither frequency facilitation is enhanced in the presence of the group II mGluR antagonist LY341495, nor is a test stimulus given after a high-frequency burst. In summary, we present pharmacological evidence for presynaptic group II mGluRs targeting subicular bursting cells, but both low- and high-frequency stimulation protocols failed to activate presynaptically located mGluRs.

KW - amino acids

KW - amino acids, dicarboxylic

KW - animals

KW - CA1 region, hippocampal

KW - CA3 region, hippocampal

KW - cyclopropanes

KW - dose-response relationship, drug

KW - excitatory amino acid agonists

KW - excitatory amino acid antagonists

KW - female

KW - glutamic acid

KW - glycine

KW - hippocampus

KW - male

KW - neurons

KW - patch-clamp techniques

KW - rats

KW - rats, wistar

KW - receptors, metabotropic glutamate

KW - synaptic transmission

KW - xanthenes

UR - http://journals.plos.org/plosone/

U2 - 10.1371/journal.pone.0045039

DO - 10.1371/journal.pone.0045039

M3 - Article

VL - 7

SP - 1

EP - 9

JO - PLOS One

T2 - PLOS One

JF - PLOS One

SN - 1932-6203

IS - 9

M1 - e45039

ER -