Group II metabotropic glutamate receptors depress synaptic transmission onto subicular burst firing neurons

Michael Kintscher, Jörg Breustedt, Stéphanie Miceli, Dietmar Schmitz, Christian Wozny

Research output: Contribution to journalArticle

5 Citations (Scopus)
35 Downloads (Pure)

Abstract

The subiculum (SUB) is a pivotal structure positioned between the hippocampus proper and various cortical and subcortical areas. Despite the growing body of anatomical and intrinsic electrophysiological data of subicular neurons, modulation of synaptic transmission in the SUB is not well understood. In the present study we investigated the role of group II metabotropic glutamate receptors (mGluRs), which have been shown to be involved in the regulation of synaptic transmission by suppressing presynaptic cAMP activity. Using field potential and patch-clamp whole cell recordings we demonstrate that glutamatergic transmission at CA1-SUB synapses is depressed by group II mGluRs in a cell-type specific manner. Application of the group II mGluR agonist (2S,1'R,2'R,3'R)-2-(2, 3-dicarboxycyclopropyl)glycine (DCG-IV) led to a significantly higher reduction of excitatory postsynaptic currents in subicular bursting cells than in regular firing cells. We further used low-frequency stimulation protocols and brief high-frequency bursts to test whether synaptically released glutamate is capable of activating presynaptic mGluRs. However, neither frequency facilitation is enhanced in the presence of the group II mGluR antagonist LY341495, nor is a test stimulus given after a high-frequency burst. In summary, we present pharmacological evidence for presynaptic group II mGluRs targeting subicular bursting cells, but both low- and high-frequency stimulation protocols failed to activate presynaptically located mGluRs.

Original languageEnglish
Article numbere45039
Pages (from-to)1-9
Number of pages9
JournalPLOS One
Volume7
Issue number9
DOIs
Publication statusPublished - 11 Sep 2012

Keywords

  • amino acids
  • amino acids, dicarboxylic
  • animals
  • CA1 region, hippocampal
  • CA3 region, hippocampal
  • cyclopropanes
  • dose-response relationship, drug
  • excitatory amino acid agonists
  • excitatory amino acid antagonists
  • female
  • glutamic acid
  • glycine
  • hippocampus
  • male
  • neurons
  • patch-clamp techniques
  • rats
  • rats, wistar
  • receptors, metabotropic glutamate
  • synaptic transmission
  • xanthenes

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