Gold nanorods for fluorescence lifetime imaging in biology

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Two-photon luminescence (TPL) from gold nanorods shows considerable potential in biological imaging. We study the imaging of gold nanorods in Madin-Darby canine kidney (MDCK) cells using fluorescence lifetime imaging microscopy (FLIM). FLIM provides images with better contrast and sensitivity than intensity imaging. The characteristic fluorescence lifetime of gold nanorods is found to be less than 100 ps, which can be used to distinguish gold nanorods from other fluorescent labels and endogenous fluorophores in lifetime imaging.
LanguageEnglish
Article number20504
Number of pages3
JournalJournal of Biomedical Optics
Volume15
Issue number2
DOIs
Publication statusPublished - 2 Apr 2010

Fingerprint

Nanorods
biology
Gold
nanorods
Fluorescence
gold
Imaging techniques
life (durability)
fluorescence
microscopy
Microscopic examination
kidneys
Fluorophores
luminescence
Luminescence
Labels
sensitivity
photons
Photons
cells

Keywords

  • two-photon luminescence
  • madin-darby canine kidney cells
  • fluorescence lifetime imaging microscopy
  • gold nanorods

Cite this

@article{a536127322f44972bffa11c6d6b69217,
title = "Gold nanorods for fluorescence lifetime imaging in biology",
abstract = "Two-photon luminescence (TPL) from gold nanorods shows considerable potential in biological imaging. We study the imaging of gold nanorods in Madin-Darby canine kidney (MDCK) cells using fluorescence lifetime imaging microscopy (FLIM). FLIM provides images with better contrast and sensitivity than intensity imaging. The characteristic fluorescence lifetime of gold nanorods is found to be less than 100 ps, which can be used to distinguish gold nanorods from other fluorescent labels and endogenous fluorophores in lifetime imaging.",
keywords = "two-photon luminescence, madin-darby canine kidney cells, fluorescence lifetime imaging microscopy, gold nanorods",
author = "Y. Zhang and Jun Yu and David Birch and Yu Chen",
year = "2010",
month = "4",
day = "2",
doi = "10.1117/1.3366646",
language = "English",
volume = "15",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
number = "2",

}

Gold nanorods for fluorescence lifetime imaging in biology. / Zhang, Y.; Yu, Jun; Birch, David; Chen, Yu.

In: Journal of Biomedical Optics, Vol. 15, No. 2, 20504, 02.04.2010.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Gold nanorods for fluorescence lifetime imaging in biology

AU - Zhang, Y.

AU - Yu, Jun

AU - Birch, David

AU - Chen, Yu

PY - 2010/4/2

Y1 - 2010/4/2

N2 - Two-photon luminescence (TPL) from gold nanorods shows considerable potential in biological imaging. We study the imaging of gold nanorods in Madin-Darby canine kidney (MDCK) cells using fluorescence lifetime imaging microscopy (FLIM). FLIM provides images with better contrast and sensitivity than intensity imaging. The characteristic fluorescence lifetime of gold nanorods is found to be less than 100 ps, which can be used to distinguish gold nanorods from other fluorescent labels and endogenous fluorophores in lifetime imaging.

AB - Two-photon luminescence (TPL) from gold nanorods shows considerable potential in biological imaging. We study the imaging of gold nanorods in Madin-Darby canine kidney (MDCK) cells using fluorescence lifetime imaging microscopy (FLIM). FLIM provides images with better contrast and sensitivity than intensity imaging. The characteristic fluorescence lifetime of gold nanorods is found to be less than 100 ps, which can be used to distinguish gold nanorods from other fluorescent labels and endogenous fluorophores in lifetime imaging.

KW - two-photon luminescence

KW - madin-darby canine kidney cells

KW - fluorescence lifetime imaging microscopy

KW - gold nanorods

U2 - 10.1117/1.3366646

DO - 10.1117/1.3366646

M3 - Article

VL - 15

JO - Journal of Biomedical Optics

T2 - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 2

M1 - 20504

ER -