Glycan–glycan interactions determine Leishmania attachment to the midgut of permissive sand fly vectors

Amy R. Hall, Jamie T. Blakeman, Ahmed M. Eissa, Paul Chapman, Ana L. Morales-Garcia, Laura Stennett, Oihane Martin, Emilie Giraud, David H. Dockrell, Neil R. Cameron, Martin Wiese, Laith Yakop, Matthew E. Rogers, Mark Geoghegan

Research output: Contribution to journalArticle

Abstract

Direct glycan–glycan interactions are increasingly implicated in survival and pathogenicity of bacteria. Here, we show that they can be exploited by protozoan parasites in their insect hosts. Force spectroscopy revealed that Leishmania promastigotes display a high-affinity biomolecular interaction between their lipophosphoglycan glycocalyx and mimics of N-acetyl-D-galactosamine, commonly expressed on the midguts of a wide range of sand fly vector species. This enabled gut-adhesive nectomonad promastigotes of Leishmania mexicana to efficiently bind to membrane-bound mucin-like, O-linked glycoproteins of the sand fly Lutzomyia longipalpis, an event crucial for parasite survival, and accounts for a permissive mode of binding. Thus, direct interaction between parasite and sand fly midgut glycans are key to permitting vector competence for all forms of leishmaniasis worldwide. In addition, these studies demonstrate the feasibility of interfering with these interactions as transmission-blocking vaccines.
Original languageEnglish
Number of pages11
JournalChemical Science
Early online date3 Sep 2020
DOIs
Publication statusE-pub ahead of print - 3 Sep 2020

Keywords

  • glycan–glycan interactions
  • Leishmania mexicana
  • Lutzomyia longipalpis

Fingerprint Dive into the research topics of 'Glycan–glycan interactions determine Leishmania attachment to the midgut of permissive sand fly vectors'. Together they form a unique fingerprint.

  • Cite this

    Hall, A. R., Blakeman, J. T., Eissa, A. M., Chapman, P., Morales-Garcia, A. L., Stennett, L., Martin, O., Giraud, E., Dockrell, D. H., Cameron, N. R., Wiese, M., Yakop, L., Rogers, M. E., & Geoghegan, M. (2020). Glycan–glycan interactions determine Leishmania attachment to the midgut of permissive sand fly vectors. Chemical Science. https://doi.org/10.1039/D0SC03298K