Global phylogeny of Shigella sonnei from limited single nucleotide polymorphisms and development of a rapid and cost-effective SNP-typing scheme for strain identification by high resolution melting: rapid SNP-typing S. sonnei

Vartul Sangal, K.E. Holt, J Yuan, D.J. Brown, I. Filliol-Toutain, François-Xavier Weill, D.W. Kim, W.D. da Silveira , D. Pickard, N.R. Thomson , J. Parkhill, Jun Yu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The current Shigella sonnei pandemic involves geographically associated, multidrug resistant clones. This study has demonstrated that S. sonnei phylogeny can be accurately defined with limited SNPs. By typing 6 informative SNPs using high-resolution melting (HRM) assay major S. sonnei lineages/sublineages can be identified as defined from whole-genome variation.
LanguageEnglish
JournalJournal of Clinical Microbiology
Early online date31 Oct 2012
DOIs
Publication statusPublished - 1 Jan 2013

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Shigella sonnei
Phylogeny
Freezing
Single Nucleotide Polymorphism
Costs and Cost Analysis
Pandemics
Clone Cells
Genome

Keywords

  • global phylogeny
  • shigella sonnei
  • single nucleotide polymorphisms
  • snp-typing scheme
  • strain identification
  • high resolution
  • melting analysis

Cite this

Sangal, Vartul ; Holt, K.E. ; Yuan, J ; Brown, D.J. ; Filliol-Toutain, I. ; Weill, François-Xavier ; Kim, D.W. ; da Silveira , W.D. ; Pickard, D. ; Thomson , N.R. ; Parkhill, J. ; Yu, Jun. / Global phylogeny of Shigella sonnei from limited single nucleotide polymorphisms and development of a rapid and cost-effective SNP-typing scheme for strain identification by high resolution melting : rapid SNP-typing S. sonnei. In: Journal of Clinical Microbiology. 2013.
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Global phylogeny of Shigella sonnei from limited single nucleotide polymorphisms and development of a rapid and cost-effective SNP-typing scheme for strain identification by high resolution melting : rapid SNP-typing S. sonnei. / Sangal, Vartul; Holt, K.E.; Yuan, J; Brown, D.J.; Filliol-Toutain, I.; Weill, François-Xavier ; Kim, D.W.; da Silveira , W.D.; Pickard, D.; Thomson , N.R.; Parkhill, J.; Yu, Jun.

In: Journal of Clinical Microbiology, 01.01.2013.

Research output: Contribution to journalArticle

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