Global phylogeny of Shigella sonnei from limited single nucleotide polymorphisms and development of a rapid and cost-effective SNP-typing scheme for strain identification by high resolution melting: rapid SNP-typing S. sonnei

Vartul Sangal, K.E. Holt, J Yuan, D.J. Brown, I. Filliol-Toutain, François-Xavier Weill, D.W. Kim, W.D. da Silveira , D. Pickard, N.R. Thomson , J. Parkhill, Jun Yu

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Abstract

The current Shigella sonnei pandemic involves geographically associated, multidrug resistant clones. This study has demonstrated that S. sonnei phylogeny can be accurately defined with limited SNPs. By typing 6 informative SNPs using high-resolution melting (HRM) assay major S. sonnei lineages/sublineages can be identified as defined from whole-genome variation.
Original languageEnglish
JournalJournal of Clinical Microbiology
Early online date31 Oct 2012
DOIs
Publication statusPublished - 1 Jan 2013

Keywords

  • global phylogeny
  • shigella sonnei
  • single nucleotide polymorphisms
  • snp-typing scheme
  • strain identification
  • high resolution
  • melting analysis

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