Genomic plasticity of pathogenic Escherichia coli mediates D-serine tolerance via multiple adaptive mechanisms

Nicky O'Boyle, James P. R. Connolly, Nicholas P. Tucker, Andrew J. Roe

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)
6 Downloads (Pure)

Abstract

Significance Pathogens ensure infection of favored sites in the body by responding to chemical signals. One chemical abundant in urine, the amino acid d -Ser, is toxic to EHEC and reduces expression of the machinery used for host cell attachment, making the bladder an unfavorable environment. We observed that under d -Ser stress, EHEC acquires genetic changes that lead to blocking d -Ser uptake into the cell or activating a silent enzyme for degrading d -Ser. This prevents growth inhibition and, critically, inhibits the repression of attachment machinery normally caused by d -Ser. These findings highlight the importance of pathogen evolution in determining how host molecules regulate colonization. These interactions underpin a process known as niche restriction that is important for pathogen success within the host.
Original languageEnglish
Pages (from-to)22484-22493
Number of pages10
JournalProceedings of the National Academy of Sciences of the United States of America
Volume117
Issue number36
Early online date26 Aug 2020
DOIs
Publication statusPublished - 8 Sep 2020

Keywords

  • D-serine
  • Escherichia coli
  • pathogens

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