Genome-wide identification of HrpL-regulated genes in the necrotrophic phytopathogen Dickeya dadantii 3937

Shihui Yang, Quan Peng, Qiu Zhang, Lifang Zou, Yan Li, Christelle Robert, Leighton Pritchard, Hui Liu, Raymond Hovey, Qi Wang, Paul Birch, Ian K. Toth, Ching-Hong Yang

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Background: Dickeya dadantii is a necrotrophic pathogen causing disease in many plants. Previous studies have demonstrated that the type III secretion system (T3SS) of D. dadantii is required for full virulence. HrpL is an alternative sigma factor that binds to the hrp box promoter sequence of T3SS genes to up-regulate their expression. Methodology/Principal Findings: To explore the inventory of HrpL-regulated genes of D. dadantii 3937 (3937), transcriptome profiles of wild-type 3937 and a hrpL mutant grown in a T3SS-inducing medium were examined. Using a cutoff value of 1.5, significant differential expression was observed in sixty-three genes, which are involved in various cellular functions such as type III secretion, chemotaxis, metabolism, regulation, and stress response. A hidden Markov model (HMM) was used to predict candidate hrp box binding sites in the intergenic regions of 3937, including the promoter regions of HrpL-regulated genes identified in the microarray assay. In contrast to biotrophic phytopathgens such as Pseudomonas syringae, among the HrpL up-regulated genes in 3937 only those within the T3SS were found to contain a hrp box sequence. Moreover, direct binding of purified HrpL protein to the hrp box was demonstrated for hrp box-containing DNA fragments of hrpA and hrpN using the electrophoretic mobility shift assay (EMSA). In this study, a putative T3SS effector DspA/E was also identified as a HrpL-upregulated gene, and shown to be translocated into plant cells in a T3SS-dependent manner. Conclusion/Significances: We provide the genome-wide study of HrpL-regulated genes in a necrotrophic phytopathogen (D. dadantii 3937) through a combination of transcriptomics and bioinformatics, which led to identification of several effectors. Our study indicates the extent of differences for T3SS effector protein inventory requirements between necrotrophic and biotrophic pathogens, and may allow the development of different strategies for disease control for these different groups of pathogens.

Original languageEnglish
Article numbere13472
Number of pages10
JournalPLoS ONE
Volume5
Issue number10
DOIs
Publication statusPublished - 19 Oct 2010

Keywords

  • Dickeya dadantii
  • necrotrophic pathogen
  • plant pathogen
  • HrpL-regulated genes

Cite this

Yang, S., Peng, Q., Zhang, Q., Zou, L., Li, Y., Robert, C., Pritchard, L., Liu, H., Hovey, R., Wang, Q., Birch, P., Toth, I. K., & Yang, C-H. (2010). Genome-wide identification of HrpL-regulated genes in the necrotrophic phytopathogen Dickeya dadantii 3937. PLoS ONE, 5(10), [e13472]. https://doi.org/10.1371/journal.pone.0013472