Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors

K. S. Bell; M. Sebaihia; L. Pritchard; M. T. G. Holden; L. J. Hyman; M. C. Holeva; N. R. Thomson; S. D. Bentley; L. J. C. Churcher; K. Mungall; R. Atkin; N. Bason; K. Brooks; T. Chillingworth; K. Clark; J. Doggett; A. Fraser; Z. Hance; H. Hauser; K. Jagels; S. Moule; H. Norbertczak; D. Ormond; C. Price; M. A. Quail; M. Sanders; D. Walker; S. Whitehead; G. P. C. Salmond; P. R. J. Birch; J. Parkhill; I. K. Toth

doi:10.1073/pnas.0402424101

Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors

K. S. Bell, M. Sebaihia, L. Pritchard, M. T. G. Holden, L. J. Hyman, M. C. Holeva, N. R. Thomson, S. D. Bentley, L. J. C. Churcher, K. Mungall, R. Atkin, N. Bason, K. Brooks, T. Chillingworth, K. Clark, J. Doggett, A. Fraser, Z. Hance, H. Hauser, K. JagelsS. Moule, H. Norbertczak, D. Ormond, C. Price, M. A. Quail, M. Sanders, D. Walker, S. Whitehead, G. P. C. Salmond, P. R. J. Birch, J. Parkhill^*, I. K. Toth

^*Corresponding author for this work

Strathclyde Institute Of Pharmacy And Biomedical Sciences

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350 Citations (Scopus)

Abstract

The bacterial family Enterobacteriaceae is notable for its well studied human pathogens, including Salmonella, Yersinia, Shigella, and Escherichia spp. However, it also contains several plant pathogens. We report the genome sequence of a plant pathogenic enterobacterium, Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043, the causative agent of soft rot and blackleg potato diseases. Approximately 33% of Eca genes are not shared with sequenced enterobacterial human pathogens, including some predicted to facilitate unexpected metabolic traits, such as nitrogen fixation and opine catabolism. This proportion of genes also contains an overrepresentation of pathogenicity determinants, including possible horizontally acquired gene clusters for putative type IV secretion and polyketide phytotoxin synthesis. To investigate whether these gene clusters play a role in the disease process, an arrayed set of insertional mutants was generated, and mutations were identified. Plant bioassays showed that these mutants were significantly reduced in virulence, demonstrating both the presence of novel pathogenicity determinants in Eca, and the impact of functional genomics in expanding our understanding of phytopathogenicity in the Enterobacteriaceae.

Original language	English
Pages (from-to)	11105-11110
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	101
Issue number	30
DOIs	https://doi.org/10.1073/pnas.0402424101
Publication status	Published - 27 Jul 2004

Keywords

enterobacteriaceae
plant pathogens
genome sequence

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10.1073/pnas.0402424101

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Bell, K. S., Sebaihia, M., Pritchard, L., Holden, M. T. G., Hyman, L. J., Holeva, M. C., Thomson, N. R., Bentley, S. D., Churcher, L. J. C., Mungall, K., Atkin, R., Bason, N., Brooks, K., Chillingworth, T., Clark, K., Doggett, J., Fraser, A., Hance, Z., Hauser, H., ... Toth, I. K. (2004). Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors. Proceedings of the National Academy of Sciences of the United States of America, 101(30), 11105-11110. https://doi.org/10.1073/pnas.0402424101

@article{b5a584fd3b4d4034b801c71da69fd113,

title = "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors",

abstract = "The bacterial family Enterobacteriaceae is notable for its well studied human pathogens, including Salmonella, Yersinia, Shigella, and Escherichia spp. However, it also contains several plant pathogens. We report the genome sequence of a plant pathogenic enterobacterium, Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043, the causative agent of soft rot and blackleg potato diseases. Approximately 33% of Eca genes are not shared with sequenced enterobacterial human pathogens, including some predicted to facilitate unexpected metabolic traits, such as nitrogen fixation and opine catabolism. This proportion of genes also contains an overrepresentation of pathogenicity determinants, including possible horizontally acquired gene clusters for putative type IV secretion and polyketide phytotoxin synthesis. To investigate whether these gene clusters play a role in the disease process, an arrayed set of insertional mutants was generated, and mutations were identified. Plant bioassays showed that these mutants were significantly reduced in virulence, demonstrating both the presence of novel pathogenicity determinants in Eca, and the impact of functional genomics in expanding our understanding of phytopathogenicity in the Enterobacteriaceae.",

keywords = "enterobacteriaceae, plant pathogens, genome sequence",

author = "Bell, {K. S.} and M. Sebaihia and L. Pritchard and Holden, {M. T. G.} and Hyman, {L. J.} and Holeva, {M. C.} and Thomson, {N. R.} and Bentley, {S. D.} and Churcher, {L. J. C.} and K. Mungall and R. Atkin and N. Bason and K. Brooks and T. Chillingworth and K. Clark and J. Doggett and A. Fraser and Z. Hance and H. Hauser and K. Jagels and S. Moule and H. Norbertczak and D. Ormond and C. Price and Quail, {M. A.} and M. Sanders and D. Walker and S. Whitehead and Salmond, {G. P. C.} and Birch, {P. R. J.} and J. Parkhill and Toth, {I. K.}",

year = "2004",

month = jul,

day = "27",

doi = "10.1073/pnas.0402424101",

language = "English",

volume = "101",

pages = "11105--11110",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

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Bell, KS, Sebaihia, M, Pritchard, L, Holden, MTG, Hyman, LJ, Holeva, MC, Thomson, NR, Bentley, SD, Churcher, LJC, Mungall, K, Atkin, R, Bason, N, Brooks, K, Chillingworth, T, Clark, K, Doggett, J, Fraser, A, Hance, Z, Hauser, H, Jagels, K, Moule, S, Norbertczak, H, Ormond, D, Price, C, Quail, MA, Sanders, M, Walker, D, Whitehead, S, Salmond, GPC, Birch, PRJ, Parkhill, J & Toth, IK 2004, 'Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors', Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 30, pp. 11105-11110. https://doi.org/10.1073/pnas.0402424101

Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors. / Bell, K. S.; Sebaihia, M.; Pritchard, L. et al.
In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 101, No. 30, 27.07.2004, p. 11105-11110.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors

AU - Bell, K. S.

AU - Sebaihia, M.

AU - Pritchard, L.

AU - Holden, M. T. G.

AU - Hyman, L. J.

AU - Holeva, M. C.

AU - Thomson, N. R.

AU - Bentley, S. D.

AU - Churcher, L. J. C.

AU - Mungall, K.

AU - Atkin, R.

AU - Bason, N.

AU - Brooks, K.

AU - Chillingworth, T.

AU - Clark, K.

AU - Doggett, J.

AU - Fraser, A.

AU - Hance, Z.

AU - Hauser, H.

AU - Jagels, K.

AU - Moule, S.

AU - Norbertczak, H.

AU - Ormond, D.

AU - Price, C.

AU - Quail, M. A.

AU - Sanders, M.

AU - Walker, D.

AU - Whitehead, S.

AU - Salmond, G. P. C.

AU - Birch, P. R. J.

AU - Parkhill, J.

AU - Toth, I. K.

PY - 2004/7/27

Y1 - 2004/7/27

N2 - The bacterial family Enterobacteriaceae is notable for its well studied human pathogens, including Salmonella, Yersinia, Shigella, and Escherichia spp. However, it also contains several plant pathogens. We report the genome sequence of a plant pathogenic enterobacterium, Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043, the causative agent of soft rot and blackleg potato diseases. Approximately 33% of Eca genes are not shared with sequenced enterobacterial human pathogens, including some predicted to facilitate unexpected metabolic traits, such as nitrogen fixation and opine catabolism. This proportion of genes also contains an overrepresentation of pathogenicity determinants, including possible horizontally acquired gene clusters for putative type IV secretion and polyketide phytotoxin synthesis. To investigate whether these gene clusters play a role in the disease process, an arrayed set of insertional mutants was generated, and mutations were identified. Plant bioassays showed that these mutants were significantly reduced in virulence, demonstrating both the presence of novel pathogenicity determinants in Eca, and the impact of functional genomics in expanding our understanding of phytopathogenicity in the Enterobacteriaceae.

AB - The bacterial family Enterobacteriaceae is notable for its well studied human pathogens, including Salmonella, Yersinia, Shigella, and Escherichia spp. However, it also contains several plant pathogens. We report the genome sequence of a plant pathogenic enterobacterium, Erwinia carotovora subsp. atroseptica (Eca) strain SCRI1043, the causative agent of soft rot and blackleg potato diseases. Approximately 33% of Eca genes are not shared with sequenced enterobacterial human pathogens, including some predicted to facilitate unexpected metabolic traits, such as nitrogen fixation and opine catabolism. This proportion of genes also contains an overrepresentation of pathogenicity determinants, including possible horizontally acquired gene clusters for putative type IV secretion and polyketide phytotoxin synthesis. To investigate whether these gene clusters play a role in the disease process, an arrayed set of insertional mutants was generated, and mutations were identified. Plant bioassays showed that these mutants were significantly reduced in virulence, demonstrating both the presence of novel pathogenicity determinants in Eca, and the impact of functional genomics in expanding our understanding of phytopathogenicity in the Enterobacteriaceae.

KW - enterobacteriaceae

KW - plant pathogens

KW - genome sequence

UR - http://www.scopus.com/inward/record.url?scp=3342994863&partnerID=8YFLogxK

U2 - 10.1073/pnas.0402424101

DO - 10.1073/pnas.0402424101

M3 - Article

C2 - 15263089

AN - SCOPUS:3342994863

SN - 0027-8424

VL - 101

SP - 11105

EP - 11110

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 30

ER -

Genome sequence of the enterobacterial phytopathogen Erwinia carotovora subsp. atroseptica and characterization of virulence factors

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