Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coliK12 and O157

J. Qi, J. Xu, Y. Hou, J. Yu

Research output: Contribution to journalArticle

333 Citations (Scopus)

Abstract

We have sequenced the genome of Shigella flexneri serotype 2a, the most prevalent species and serotype that causes bacillary dysentery or shigellosis in man. The whole genome is composed of a 4 607 203 bp chromosome and a 221 618 bp virulence plasmid, designated pCP301. While the plasmid shows minor divergence from that sequenced in serotype 5a, striking characteristics of the chromosome have been revealed. The S.flexneri chromosome has, astonishingly, 314 IS elements, more than 7-fold over those possessed by its close relatives, the non-pathogenic K12 strain and enterohemorrhagic O157:H7 strain of Escherichia coli. There are 13 translocations and inversions compared with the E.coli sequences, all involve a segment larger than 5 kb, and most are associated with deletions or acquired DNA sequences, of which several are likely to be bacteriophage-transmitted pathogenicity islands. Furthermore, S.flexneri, resembling another human-restricted enteric pathogen, Salmonella typhi, also has hundreds of pseudogenes compared with the E.coli strains. All of these could be subjected to investigations towards novel preventative and treatment strategies against shigellosis.
LanguageEnglish
Pages4432-41
Number of pages4391
JournalNucleic Acids Research
Volume30
Issue number20
DOIs
Publication statusPublished - 2002

Fingerprint

Bacillary Dysentery
Shigella flexneri
Escherichia
Virulence
Chromosomes
Genome
Plasmids
Escherichia coli
Genomic Islands
Salmonella typhi
Pseudogenes
DNA Transposable Elements
Escherichia coli O157
Bacteriophages
Serogroup
Therapeutics

Keywords

  • genome
  • Shigella flexneri
  • pathogenicity
  • bioscience
  • physiology

Cite this

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abstract = "We have sequenced the genome of Shigella flexneri serotype 2a, the most prevalent species and serotype that causes bacillary dysentery or shigellosis in man. The whole genome is composed of a 4 607 203 bp chromosome and a 221 618 bp virulence plasmid, designated pCP301. While the plasmid shows minor divergence from that sequenced in serotype 5a, striking characteristics of the chromosome have been revealed. The S.flexneri chromosome has, astonishingly, 314 IS elements, more than 7-fold over those possessed by its close relatives, the non-pathogenic K12 strain and enterohemorrhagic O157:H7 strain of Escherichia coli. There are 13 translocations and inversions compared with the E.coli sequences, all involve a segment larger than 5 kb, and most are associated with deletions or acquired DNA sequences, of which several are likely to be bacteriophage-transmitted pathogenicity islands. Furthermore, S.flexneri, resembling another human-restricted enteric pathogen, Salmonella typhi, also has hundreds of pseudogenes compared with the E.coli strains. All of these could be subjected to investigations towards novel preventative and treatment strategies against shigellosis.",
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Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coliK12 and O157. / Qi, J.; Xu, J.; Hou, Y.; Yu, J.

In: Nucleic Acids Research, Vol. 30, No. 20, 2002, p. 4432-41.

Research output: Contribution to journalArticle

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