Genetic immunization using liposome-incorporated DNA

Yvonne Perrie, Gregory Gregoriadis

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Genetic immunization using naked plasmid DNA is based on DNA uptake by and expression in skeletal muscle cells which lack vital co-stimulatory molecules. Alternatively liposomes, which target antigen-presenting cells directly and protect DNA from nuclease attack, Perrie & Gregoriadis, (1997) may be preferable. We have recently shown, Gregoriadis et al(1997); Gregoriadis et al(1998) that Balb/c and outbred mice immunized by a variety of routes with the plasmid DNA pRc/CMV HBS (encoding the S region of HBsAg, subtype ayw) entrapped in cationic liposomes mounted much higher humoral (IgG and splenic IL-4) and cell mediated (splenic IFN-y) responses than when injected with naked DNA or DNA complexed to similar preformed liposomes. Here we report further studies on the effect of lipid composition of cationic liposomes entrapping the plasmid, on immune responses.
LanguageEnglish
Pages103-103
Number of pages1
JournalJournal of Pharmacy and Pharmacology
Volume50
Issue numberS9
DOIs
Publication statusPublished - 30 Sep 1998

Fingerprint

Liposomes
Immunization
DNA
Plasmids
Deoxyribonucleases
Antigen-Presenting Cells
Hepatitis B Surface Antigens
Interleukin-4
Muscle Cells
Skeletal Muscle
Immunoglobulin G
Lipids

Keywords

  • genetic immunisation
  • DNA
  • liposomes
  • immune responses

Cite this

Perrie, Yvonne ; Gregoriadis, Gregory. / Genetic immunization using liposome-incorporated DNA. In: Journal of Pharmacy and Pharmacology. 1998 ; Vol. 50, No. S9. pp. 103-103.
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Genetic immunization using liposome-incorporated DNA. / Perrie, Yvonne; Gregoriadis, Gregory.

In: Journal of Pharmacy and Pharmacology, Vol. 50, No. S9, 30.09.1998, p. 103-103.

Research output: Contribution to journalArticle

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