Genetic and cytological methods to study ESCRT cell cycle function in fission yeast

Imane M. Rezig, Shaun K. Bremner, Musab S. Bhutta, Ian P. Salt, Gwyn W. Gould, Christopher J. McInerny

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The fission yeast Schizosaccharomyces pombe, an ascomycete fungus, is an established model organism for studying eukaryotic molecular and cellular events such as the cell cycle due to its powerful genetics, a sequenced genome, and the ease of molecular manipulation (Wood et al., Nature 415:871–880, 2002; Hoffman et al., Genetics 201:403–423, 2015). This chapter describes genetic and cytological methods to study endosomal sorting complex required for transport (ESCRT) function during the cell cycle in fission yeast. These include tetrad analysis to allow the creation of double mutants to test for genetic interactions by synthetic phenotype characterization, such as cellular growth and the analysis of division septa by calcofluor-white staining.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Place of PublicationNew York, NY.
Pages239-250
Number of pages12
DOIs
Publication statusPublished - 28 Jun 2019

Publication series

NameMethods in Molecular Biology
Volume1998
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Anillin
  • Calcofluor-white
  • ESCRT
  • Fission yeast
  • Genetic interaction
  • Polo kinase
  • Schizosaccharomyces pombe
  • Septation
  • Synthetic phenotype
  • Tetrad analysis

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